Reference - Detail
| RRC ID | 54345 |
|---|---|
| Author | Nakahashi Y, Taketani S, Okuda M, Inoue K, Tokunaga R. |
| Title | Molecular cloning and sequence analysis of cDNA encoding human ferrochelatase. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
The cDNA encoding human ferrochelatase [EC 4.99.1.1] was isolated from a human placenta cDNA library in bacteriophage lambda gt11 by screening with a radiolabeled fragment of mouse ferrochelatase cDNA. The cDNA had an open reading frame of 1269 base pairs (bp) encoding a protein of 423 amino acid residues (Mr. 47,833) with alternative putative polyadenylation signals in the 3' non-coding regions and poly (A) tails. Amino acid sequencing showed that the mature protein consists of 369 amino acid residues (Mr. 42,158) with a putative leader sequence of 54 amino acid residues. The human enzyme showed an 88% identity to mouse enzyme and 46% to yeast enzyme. Northern blot analysis showed two mRNAs of about 2500 and 1600 bp for ferrochelatase in K562 and HepG2 cells. As full-length cDNA for human ferrochelatase is now available, molecular lesions related to erythropoietic protoporphyria can be characterized. |
| Volume | 173(2) |
| Pages | 748-55 |
| Published | 1990-12-14 |
| DOI | 10.1016/s0006-291x(05)80099-3 |
| PII | S0006-291X(05)80099-3 |
| PMID | 2260980 |
| MeSH | Amino Acid Sequence Animals Blotting, Northern Cattle Cloning, Molecular DNA / biosynthesis* Ferrochelatase / biosynthesis Ferrochelatase / genetics* Humans Mice Molecular Sequence Data Open Reading Frames RNA, Messenger / chemistry Restriction Mapping Sequence Homology, Nucleic Acid Tumor Cells, Cultured |
| IF | 2.985 |
| Times Cited | 148 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | ferrochelatase (RDB01377) |