論文 - 詳細
| RRC ID | 54354 |
|---|---|
| 著者 | Kobayashi S, Hara A, Isagawa T, Manabe I, Takeda K, MaruYama T. |
| タイトル | The nuclear IκB family protein IκBNS influences the susceptibility to experimental autoimmune encephalomyelitis in a murine model. |
| ジャーナル | PLoS One |
| Abstract |
The nuclear IκB family protein IκBNS is expressed in T cells and plays an important role in Interferon (IFN)-γ and Interleukin (IL)-2 production. IκB-ζ, the most similar homolog of IκBNS, plays an important role in the generation of T helper (Th)17 cells in cooperation with RORγt, a master regulator of Th17 cells. Thus, IκB-ζ deficient mice are resistant to Th17-dependent experimental autoimmune encephalomyelitis (EAE). However, IκB-ζ deficient mice develop the autoimmune-like Sjögren syndrome with aging. Here we found that IκBNS-deficient (Nfkbid-/-) mice show resistance against developing Th17-dependent EAE. We found that Nfkbid-/- T cells have decreased expression of IL-17-related genes and RORγt in response to Transforming Growth Factor (TGF)-β1 and IL-6 stimulation. Thus, IκBNS plays a pivotal role in the generation of Th17 cells and in the control of Th17-dependent EAE. |
| 巻・号 | 9(10) |
| ページ | e110838 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0110838 |
| PII | PONE-D-14-24165 |
| PMID | 25347393 |
| PMC | PMC4210207 |
| MeSH | Animals Cell Differentiation Disease Models, Animal Disease Susceptibility* Encephalomyelitis, Autoimmune, Experimental / genetics Encephalomyelitis, Autoimmune, Experimental / immunology Encephalomyelitis, Autoimmune, Experimental / metabolism* Gene Expression Regulation I-kappa B Proteins / genetics I-kappa B Proteins / metabolism* Mice Mice, Knockout NF-kappa B / genetics Nuclear Receptor Subfamily 1, Group F, Member 3 / genetics Nuclear Receptor Subfamily 1, Group F, Member 3 / metabolism T-Lymphocyte Subsets / cytology T-Lymphocyte Subsets / immunology T-Lymphocyte Subsets / metabolism Th17 Cells / cytology Th17 Cells / immunology Th17 Cells / metabolism |
| IF | 2.74 |
| 引用数 | 20 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pGL4.12-mouse IL-17A promoter (-6647 to +1) (RDB16724) pcDNA3-FLAG-mouse ROR gamma t(RDB16728) pEF6-FLAG-mouse IkappaBNS (RDB16725) pEF6-HA-mouse IkappaBNS (RDB16726) pEF6-Myc-mouse IkappaBNS (RDB16727) |