論文 - 詳細
| RRC ID | 54357 |
|---|---|
| 著者 | MaruYama T, Sayama A, Ishii KJ, Muta T. |
| タイトル | Screening of posttranscriptional regulatory molecules of IκB-ζ. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Posttranscriptional regulatory elements in the 3'-untranslated region (UTR) of mRNAs play an important role in mRNA stabilization. Induction of IκB-ζ, a critical transcriptional regulator in the innate immune response, is mediated via specific mRNA stabilization by lipopolysaccharide (LPS) and interleukin (IL)-1β. It is known that the 3'-UTR of IκB-ζ, especially 165 nucleotides after the stop codon, plays a crucial role in mRNA stability. Herein, we show that AU-rich elements and miRNA targets in these 165 3'-UTR nucleotides are dispensable for stability of IκB-ζ mRNA. Additionally, NF-κB activation is important for IκB-ζ transcription, but dispensable for IκB-ζ mRNA stability. Interestingly, high-throughput screening results show that MyD88, a signal molecule responsive to LPS/IL-1β stimulation, is key for stabilizing IκB-ζ mRNA expression. Moreover, MyD88-deficient macrophages exhibited a decreased half-life of IκB-ζ mRNA expression. These results indicate that the LPS/IL-1β-MyD88 axis plays a crucial role for stabilization of IκB-ζ mRNA. |
| 巻・号 | 469(3) |
| ページ | 711-5 |
| 公開日 | 2016-1-15 |
| DOI | 10.1016/j.bbrc.2015.12.068 |
| PII | S0006-291X(15)31073-1 |
| PMID | 26711529 |
| MeSH | Adaptor Proteins, Signal Transducing Animals Base Sequence HEK293 Cells Humans I-kappa B Proteins / genetics* Interleukin-1beta / genetics* Mice Molecular Sequence Data Myeloid Differentiation Factor 88 / genetics* Nuclear Proteins / genetics* Peptide Fragments / genetics* RAW 264.7 Cells RNA Processing, Post-Transcriptional / genetics* RNA Stability / genetics* Regulatory Elements, Transcriptional / genetics* |
| IF | 2.985 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pcDNA3-Myc-mouse MyD88N (RDB16740) pGL4.12 SV40-mouse IkBzeta 3`UTR (full) (RDB16738) pGL4.12 SV40-mouse IkBzeta 3`UTR (165nt) (RDB16739) |