論文 - 詳細
| RRC ID | 54368 |
|---|---|
| 著者 | Ou B, Yin KQ, Liu SN, Yang Y, Gu T, Wing Hui JM, Zhang L, Miao J, Kondou Y, Matsui M, Gu HY, Qu LJ. |
| タイトル | A high-throughput screening system for Arabidopsis transcription factors and its application to Med25-dependent transcriptional regulation. |
| ジャーナル | Mol Plant |
| Abstract |
The activities of transcription factors (TFs) require interactions with specific DNA sequences and other regulatory proteins. To detect such interactions in Arabidopsis, we developed a high-throughput screening system with a Gateway-compatible Gal4-AD-TF library of 1589 Arabidopsis TFs, which can be easily screened by mating-based yeast-one-hybrid (Y1H) and yeast-two-hybrid (Y2H) methods. The efficiency of the system was validated by examining two well-characterized TF-DNA and TF-protein interactions: the CHE-CCA1 promoter interaction by Y1H and NPR1-TGAs interactions by Y2H. We used this system to identify eight TFs that interact with a Mediator subunit, Med25, a key regulator in JA signaling. We identified five TFs that interacted with the GCC-box cis-element in the promoter of PDF1.2, a downstream gene of Med25. We found that three of these TFs, all from the AP2-EREBP family, interact directly both with Med25 and the GCC-box of PDF1.2, suggesting that Med25 regulates PDF1.2 expression through these three TFs. These results demonstrate that this high-throughput Y1H/Y2H screening system is an efficient tool for studying transcriptional regulation networks in Arabidopsis. This system will be available for other Arabidopsis researchers, and thus it provides a vital resource for the Arabidopsis community. |
| 巻・号 | 4(3) |
| ページ | 546-55 |
| 公開日 | 2011-5-1 |
| DOI | 10.1093/mp/ssr002 |
| PII | S1674-2052(14)60586-4 |
| PMID | 21343311 |
| MeSH | Arabidopsis / genetics* Arabidopsis / metabolism* Arabidopsis Proteins / metabolism* DNA-Binding Proteins Gene Expression Regulation, Plant* Gene Library High-Throughput Screening Assays / methods* Models, Biological Nuclear Proteins / metabolism* Promoter Regions, Genetic / genetics Protein Binding Reproducibility of Results Transcription Factors / metabolism* Transcription, Genetic* |
| IF | 12.084 |
| 引用数 | 91 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda01393 |