RRC ID |
54369
|
Author |
Yin Y, Borges G, Sakuta M, Crozier A, Ashihara H.
|
Title |
Effect of phosphate deficiency on the content and biosynthesis of anthocyanins and the expression of related genes in suspension-cultured grape (Vitis sp.) cells.
|
Journal |
Plant Physiol Biochem
|
Abstract |
The effect of phosphate (Pi) starvation on anthocyanin biosynthesis was examined in suspension-cultured grape cells derived from Bailey Alicante A grapes. Cyanindin-3-O-glucoside, peonidin-3-O-glucoside, cyanidin-3-O-(6-O״-p-coumaroyl)-glucoside and peonidin-3-O-(6-O״-p-coumaroyl)-glucoside were detected in cells in both the complete (+Pi)- and Pi-deficient Murashige-Skoog (-Pi) medium but accumulation was much more substantial cells in -Pi cultures. Highest incorporation of [ring-(14)C]phenylalanine into anthocyanins was found at days 3-14 in the cells in -Pi medium. Time-course expression profiles of anthocyanin biosynthetic enzyme genes and a regulatory gene were examined after cells were transferred to the +Pi and -Pi medium. The transcript levels of UFGT and VvmybA1 in -Pi cells were higher than in +Pi cells 24 h after transfer coinciding with the start of anthocyanin accumulation in -Pi cells.
|
Volume |
55
|
Pages |
77-84
|
Published |
2012-6-1
|
DOI |
10.1016/j.plaphy.2012.03.009
|
PII |
S0981-9428(12)00066-6
|
PMID |
22554830
|
MeSH |
Anthocyanins / biosynthesis*
Cells, Cultured
Chromatography, High Pressure Liquid
Culture Media / metabolism
Culture Media / pharmacology
Gene Expression Regulation, Plant / drug effects*
Glucosyltransferases / genetics
Glucosyltransferases / metabolism
Mass Spectrometry
Phenylalanine / metabolism
Phosphates / metabolism
Phosphates / pharmacology*
Plant Proteins / genetics*
Plant Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Vitis / cytology
Vitis / genetics
Vitis / metabolism
|
IF |
3.72
|
Times Cited |
12
|
Resource |
Arabidopsis / Cultured plant cells, genes |
rpc00003 |