論文 - 詳細
| RRC ID | 54414 |
|---|---|
| 著者 | Memisoglu G, Eapen VV, Yang Y, Klionsky DJ, Haber JE. |
| タイトル | PP2C phosphatases promote autophagy by dephosphorylation of the Atg1 complex. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Macroautophagy is orchestrated by the Atg1-Atg13 complex in budding yeast. Under nutrient-rich conditions, Atg13 is maintained in a hyperphosphorylated state by the TORC1 kinase. After nutrient starvation, Atg13 is dephosphorylated, triggering Atg1 kinase activity and macroautophagy induction. The phosphatases that dephosphorylate Atg13 remain uncharacterized. Here, we show that two redundant PP2C phosphatases, Ptc2 and Ptc3, regulate macroautophagy by dephosphorylating Atg13 and Atg1. In the absence of these phosphatases, starvation-induced macroautophagy and the cytoplasm-to-vacuole targeting pathway are inhibited, and the recruitment of the essential autophagy machinery to the phagophore assembly site is impaired. Expressing a genomic ATG13-8SA allele lacking key TORC1 phosphorylation sites partially bypasses the macroautophagy defect in ptc2Δ ptc3Δ strains. Moreover, Ptc2 and Ptc3 interact with the Atg1-Atg13 complex. Taken together, these results suggest that PP2C-type phosphatases promote macroautophagy by regulating the Atg1 complex. |
| 巻・号 | 116(5) |
| ページ | 1613-1620 |
| 公開日 | 2019-1-29 |
| DOI | 10.1073/pnas.1817078116 |
| PII | 1817078116 |
| PMID | 30655342 |
| PMC | PMC6358665 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism Autophagy / physiology* Autophagy-Related Proteins / metabolism* Phosphoric Monoester Hydrolases / metabolism* Phosphorylation / physiology Protein Kinases / metabolism* Protein Phosphatase 2C / metabolism* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / metabolism* |
| IF | 9.58 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | pRS316[GFPATG8], pRS416GAL1[ATG13], pRS416GAL1[ATG13-8SA], pRS315[mCherry-ATG8] |