RRC ID 54426
Author Shoji T, Hashimoto T.
Title Expression of a tobacco nicotine biosynthesis gene depends on the JRE4 transcription factor in heterogenous tomato.
Journal J Plant Res
Abstract The jasmonate-responsive transcription factor ERF189 in tobacco (Nicotiana tabacum) and its ortholog JRE4 in tomato (Solanum lycopersicum) regulate a series of biosynthetic genes involved in the nicotine and steroidal glycoalkaloid pathways. In tobacco, QUINOLINATE PHOSPHORIBOSYL TRANSFERASE 2 (NtQPT2) is regulated by ERF189; however, we found that the tomato QPT gene is not regulated by JRE4. Here, we explored whether and how NtQPT2 is regulated in a heterogenous tomato host. We used a NtQPT2 promoter-driven reporter gene to examine the cell type-specific and jasmonate-induced expression of this gene in transgenic tomato hairy roots. The downregulation of the reporter in the jre4 loss-of-function tomato mutant and its transactivation by JRE4 in transient expression experiments suggested that JRE4, like its ortholog ERF189 in tobacco, activates the NtQPT2 promoter in tomato. We discuss the evolution of QPT2 in the Nicotiana lineage, which mainly occurred through mutational changes in the promoter that altered the control of the functionally conserved transcription factors.
Volume 132(2)
Pages 173-180
Published 2019-3-1
DOI 10.1007/s10265-018-1075-0
PII 10.1007/s10265-018-1075-0
PMID 30478481
MeSH Evolution, Molecular Gene Expression Regulation, Plant* Genes, Reporter Lycopersicon esculentum / genetics Lycopersicon esculentum / metabolism* Nicotine / biosynthesis Pentosyltransferases / genetics* Pentosyltransferases / metabolism Plant Roots / metabolism Plants, Genetically Modified Promoter Regions, Genetic Tobacco / genetics* Transcription Factors / metabolism* Transcriptional Activation
IF 2.0
Times Cited 2
Resource
Tomato TOMJPF00001