| RRC ID |
5443
|
| 著者 |
Okazaki M, Ohkusu K, Hata H, Ohnishi H, Sugahara K, Kawamura C, Fujiwara N, Matsumoto S, Nishiuchi Y, Toyoda K, Saito H, Yonetani S, Fukugawa Y, Yamamoto M, Wada H, Sejimo A, Ebina A, Goto H, Ezaki T, Watanabe T.
|
| タイトル |
Mycobacterium kyorinense sp. nov., a novel, slow-growing species, related to Mycobacterium celatum, isolated from human clinical specimens.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel, non-pigmented, slow-growing mycobacterium was identified on the basis of biochemical and nucleic acid analyses, as well as growth characteristics. Three isolates were cultured from clinical samples (two from sputum and one from pus in lymph nodes) obtained from three immunocompetent patients with infections. Bacterial growth occurred at 28-42 degrees C on Middlebrook 7H11-OADC agar. The isolates showed negative results for Tween hydrolysis, nitrate reductase, semiquantitative catalase, urease activity, 3 day arylsulfatase activity, pyrazinamidase, tellurite reduction and niacin accumulation tests, but positive results for 14 day arylsulfatase activity and heat-stable catalase tests. The isolates contained alpha-, keto-, and dicarboxymycolates in their cell walls. Sequence analysis revealed that all isolates had identical, unique 16S rRNA sequences. Phylogenetic analysis of the 16S rRNA, rpoB, hsp65 and sodA gene sequences confirmed that these isolates are unique but closely related to Mycobacterium celatum. DNA-DNA hybridization of the isolates demonstrated less than 50 % reassociation with M. celatum and Mycobacterium branderi. On the basis of these findings, a novel species designated Mycobacterium kyorinense sp. nov. is proposed. The type strain is KUM 060204(T) (=JCM 15038(T)=DSM 45166(T)).
|
| 巻・号 |
59(Pt 6)
|
| ページ |
1336-41
|
| 公開日 |
2009-6-1
|
| DOI |
10.1099/ijs.0.000760-0
|
| PII |
59/6/1336
|
| PMID |
19502312
|
| MeSH |
Aged
Bacterial Proteins / genetics
Bacterial Typing Techniques
DNA, Bacterial / analysis
DNA, Bacterial / isolation & purification
Female
Humans
Japan
Male
Microbial Sensitivity Tests
Middle Aged
Molecular Sequence Data
Mycobacterium / classification*
Mycobacterium / genetics
Mycobacterium / growth & development*
Mycobacterium / isolation & purification
Mycobacterium Infections / microbiology*
Mycolic Acids / analysis
Nucleic Acid Hybridization
Phenotype
Phylogeny
Pneumonia, Bacterial / microbiology*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Species Specificity
Sputum / microbiology
|
| IF |
2.415
|
| 引用数 |
22
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM15038 |
