| RRC ID |
54439
|
| 著者 |
Suzaki T, Takeda N, Nishida H, Hoshino M, Ito M, Misawa F, Handa Y, Miura K, Kawaguchi M.
|
| タイトル |
LACK OF SYMBIONT ACCOMMODATION controls intracellular symbiont accommodation in root nodule and arbuscular mycorrhizal symbiosis in Lotus japonicus.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
Nitrogen-fixing rhizobia and arbuscular mycorrhizal fungi (AMF) form symbioses with plant roots and these are established by precise regulation of symbiont accommodation within host plant cells. In model legumes such as Lotus japonicus and Medicago truncatula, rhizobia enter into roots through an intracellular invasion system that depends on the formation of a root-hair infection thread (IT). While IT-mediated intracellular rhizobia invasion is thought to be the most evolutionarily derived invasion system, some studies have indicated that a basal intercellular invasion system can replace it when some nodulation-related factors are genetically modified. In addition, intracellular rhizobia accommodation is suggested to have a similar mechanism as AMF accommodation. Nevertheless, our understanding of the underlying genetic mechanisms is incomplete. Here we identify a L. japonicus nodulation-deficient mutant, with a mutation in the LACK OF SYMBIONT ACCOMMODATION (LAN) gene, in which root-hair IT formation is strongly reduced, but intercellular rhizobial invasion eventually results in functional nodule formation. LjLAN encodes a protein that is homologous to Arabidopsis MEDIATOR 2/29/32 possibly acting as a subunit of a Mediator complex, a multiprotein complex required for gene transcription. We also show that LjLAN acts in parallel with a signaling pathway including LjCYCLOPS. In addition, the lan mutation drastically reduces the colonization levels of AMF. Taken together, our data provide a new factor that has a common role in symbiont accommodation process during root nodule and AM symbiosis.
|
| 巻・号 |
15(1)
|
| ページ |
e1007865
|
| 公開日 |
2019-1-1
|
| DOI |
10.1371/journal.pgen.1007865
|
| PII |
PGENETICS-D-18-01183
|
| PMID |
30605473
|
| PMC |
PMC6317779
|
| MeSH |
Gene Expression Regulation, Plant / genetics
Lotus / genetics*
Lotus / growth & development
Lotus / microbiology
Medicago truncatula / genetics*
Medicago truncatula / growth & development
Medicago truncatula / microbiology
Mutation
Mycorrhizae / genetics
Mycorrhizae / growth & development*
Plant Proteins / genetics
Plant Root Nodulation / genetics
Plant Roots / genetics
Plant Roots / growth & development
Plant Roots / microbiology
Rhizobium / genetics
Rhizobium / growth & development
Root Nodules, Plant / genetics
Root Nodules, Plant / growth & development
Root Nodules, Plant / microbiology
Symbiosis / genetics*
|
| IF |
5.175
|
| 引用数 |
5
|
| リソース情報 |
| ミヤコグサ・ダイズ |
M. loti nodC mutant |
