Inoue N, Fukui A, Nomura M, Matsumoto M, Nishizawa Y, Toyoshima K, Seya T.
A novel chicken membrane-associated complement regulatory protein: molecular cloning and functional characterization.
A cDNA encoding a membrane-associated complement (C) regulatory protein was identified here for the first time in an oviparous vertebrate, chicken. This protein, named Cremp, possessed five short consensus repeats (SCRs) and one SCR-like domain followed by a transmembrane domain and a cytoplasmic tail. SCR1/SCR2 of Cremp were 43.6% identical with SCR2/SCR3 of human decay-accelerating factor (CD55), and SCR3/SCR4 were 45.3% identical with those of human membrane cofactor protein (CD46). Cremp is likely to be an ancestral hybrid protein of human decay-accelerating factor and membrane cofactor protein rather than a homolog of rodent C receptor 1-related protein y, which structurally resembles human CR1 (CD35). Chinese hamster ovary cells transfected with Cremp were efficiently protected from chicken C but not from human or rabbit C in both classical and alternative pathways. Thus, chicken Cremp is a membrane C regulator for cell protection against homologous C. Cremp mRNA was seen as a doublet comprised of a faint band of 2.2 kb and a thick band of 3.0 kb on RNA blotting analysis. An Ab against chicken Cremp recognized a single band of 46.8 kDa on immunoblotting. mRNA and protein of Cremp were ubiquitously expressed in all chicken organs tested. Minute amounts of dimer were present in some tissues. Surface expression of Cremp was confirmed by flow cytometry and immunofluorescence analysis. These results suggested that even in nonmammals a C regulatory membrane protein with ubiquitous tissue distribution should be a prerequisite for protection of host cells from homologous C attack.
Amino Acid Sequence
Cell Membrane / genetics
Cell Membrane / immunology
Cell Membrane / metabolism
Complement Inactivator Proteins / biosynthesis
Complement Inactivator Proteins / chemistry
Complement Inactivator Proteins / genetics*
Complement Inactivator Proteins / immunology*
Complement Pathway, Alternative / genetics
Complement Pathway, Alternative / immunology
Complement Pathway, Classical / genetics
Complement Pathway, Classical / immunology
Cytotoxicity Tests, Immunologic
DNA, Complementary / isolation & purification
Membrane Proteins / biosynthesis
Membrane Proteins / chemistry
Membrane Proteins / genetics*
Membrane Proteins / immunology*
Molecular Sequence Data
RNA, Messenger / biosynthesis
|Human and Animal Cells