RRC ID 54471
Author Heimann G, Sirko S.
Title Investigating Age-Related Changes in Proliferation and the Cell Division Repertoire of Parenchymal Reactive Astrocytes.
Journal Methods Mol Biol
Abstract Reactive gliosis is a complicated process involving all types of glial cells and is the therapeutic target of efforts to treat several types of neuropathologies. Parenchymal astrocytes continuously survey their microenvironment to identify even tiny abnormalities in the central nervous system (CNS) homeostasis and react rapidly to brain damage, such as following ischemia, trauma, or neurodegenerative diseases, to prevent propagation of tissue damage. Aging can play causal roles in certain astroglial dysfunctions, however, still little is known to what extent the heterogeneous reaction of astrocytes at the injury site might be impaired over the course of aging. Based on our experience with both in vitro and in vivo experimental paradigms, we describe here in detail the analysis of age-related changes in (1) proliferative response of parenchymal astrocytes within the posttraumatic cerebral cortex grey matter (GM), and (2) repertoire of their cell divisions in adherent cell culture prepared from the injured GM of young and old double transgenic GFAP-mRFP1/(FUCCI)-S/G2/M-mAG-hGeminin mice by single cell time-lapse imaging.
Volume 1938
Pages 277-292
Published 2019-1-9
DOI 10.1007/978-1-4939-9068-9_20
PMID 30617988
MeSH Age Factors Aging / metabolism* Animals Astrocytes / metabolism* Biomarkers Brain Injuries / etiology Brain Injuries / metabolism Brain Injuries / pathology Cell Cycle Cell Division* Cell Proliferation Cells, Cultured Cerebral Cortex / metabolism Cerebral Cortex / pathology Disease Models, Animal Female Immunohistochemistry Male Mice Molecular Imaging
Mice RBRC02706