Reference - Detail
| RRC ID | 54493 |
|---|---|
| Author | Grima B, Papin C, Martin B, Chélot E, Ponien P, Jacquet E, Rouyer F. |
| Title | PERIOD-controlled deadenylation of the timeless transcript in the Drosophila circadian clock. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
The Drosophila circadian oscillator relies on a negative transcriptional feedback loop, in which the PERIOD (PER) and TIMELESS (TIM) proteins repress the expression of their own gene by inhibiting the activity of the CLOCK (CLK) and CYCLE (CYC) transcription factors. A series of posttranslational modifications contribute to the oscillations of the PER and TIM proteins but few posttranscriptional mechanisms have been described that affect mRNA stability. Here we report that down-regulation of the POP2 deadenylase, a key component of the CCR4-NOT deadenylation complex, alters behavioral rhythms. Down-regulating POP2 specifically increases TIM protein and tim mRNA but not tim pre-mRNA, supporting a posttranscriptional role. Indeed, reduced POP2 levels induce a lengthening of tim mRNA poly(A) tail. Surprisingly, such effects are lost in per0
mutants, supporting a PER-dependent inhibition of tim mRNA deadenylation by POP2. We report a deadenylation mechanism that controls the oscillations of a core clock gene transcript. |
| Volume | 116(12) |
| Pages | 5721-5726 |
| Published | 2019-3-19 |
| DOI | 10.1073/pnas.1814418116 |
| PII | 1814418116 |
| PMID | 30833404 |
| PMC | PMC6431209 |
| MeSH | ARNTL Transcription Factors / genetics Animals CLOCK Proteins / genetics Circadian Clocks / genetics Circadian Clocks / physiology* Circadian Rhythm / physiology Down-Regulation Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila Proteins / physiology* Drosophila melanogaster / genetics Gene Expression Regulation Period Circadian Proteins / metabolism Period Circadian Proteins / physiology* Phosphorylation RNA, Messenger / metabolism Ribonucleases Transcription, Genetic |
| IF | 9.58 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 5684R-1 1884R-1 |