RRC ID 54529
Author Ayabe S, Nakashima K, Yoshiki A.
Title Off- and on-target effects of genome editing in mouse embryos.
Journal J Reprod Dev
Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome. Unbiased whole genome sequencing comparing parents to progeny has revealed that the rate of Cas9-induced mutagenesis in mouse embryos is indistinguishable from the background rate of de novo mutation. However, establishing the best practice to confirm on-target alleles of interest remains a challenge. We believe that improvement in editing strategies and screening methods for founder mice will contribute to the generation of quality-controlled animals, thereby ensuring reproducibility of results in animal studies and advancing the 3Rs (replacement, reduction, and refinement).
Volume 65(1)
Pages 1-5
Published 2019-2-8
DOI 10.1262/jrd.2018-128
PMID 30518723
PMC PMC6379761
MeSH Animals Animals, Genetically Modified / genetics CRISPR-Associated Protein 9 / genetics* CRISPR-Cas Systems / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / genetics Embryo, Mammalian* Gene Editing / methods* Mice Mice, Inbred C57BL Mutagenesis* Reproducibility of Results Whole Genome Sequencing
IF 1.635
Times Cited 6
DNA material T7-NLS hCas9-pA (RDB13130)