Reference - Detail
| RRC ID | 54529 |
|---|---|
| Author | Ayabe S, Nakashima K, Yoshiki A. |
| Title | Off- and on-target effects of genome editing in mouse embryos. |
| Journal | J Reprod Dev |
| Abstract |
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome. Unbiased whole genome sequencing comparing parents to progeny has revealed that the rate of Cas9-induced mutagenesis in mouse embryos is indistinguishable from the background rate of de novo mutation. However, establishing the best practice to confirm on-target alleles of interest remains a challenge. We believe that improvement in editing strategies and screening methods for founder mice will contribute to the generation of quality-controlled animals, thereby ensuring reproducibility of results in animal studies and advancing the 3Rs (replacement, reduction, and refinement). |
| Volume | 65(1) |
| Pages | 1-5 |
| Published | 2019-2-8 |
| DOI | 10.1262/jrd.2018-128 |
| PMID | 30518723 |
| PMC | PMC6379761 |
| MeSH | Animals Animals, Genetically Modified / genetics CRISPR-Associated Protein 9 / genetics* CRISPR-Cas Systems / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / genetics Embryo, Mammalian* Gene Editing / methods* Mice Mice, Inbred C57BL Mutagenesis* Reproducibility of Results Whole Genome Sequencing |
| IF | 1.635 |
| Times Cited | 6 |
| Resource | |
| DNA material | T7-NLS hCas9-pA (RDB13130) |