論文 - 詳細
RRC ID | 54529 |
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著者 | Ayabe S, Nakashima K, Yoshiki A. |
タイトル | Off- and on-target effects of genome editing in mouse embryos. |
ジャーナル | J Reprod Dev |
Abstract |
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome. Unbiased whole genome sequencing comparing parents to progeny has revealed that the rate of Cas9-induced mutagenesis in mouse embryos is indistinguishable from the background rate of de novo mutation. However, establishing the best practice to confirm on-target alleles of interest remains a challenge. We believe that improvement in editing strategies and screening methods for founder mice will contribute to the generation of quality-controlled animals, thereby ensuring reproducibility of results in animal studies and advancing the 3Rs (replacement, reduction, and refinement). |
巻・号 | 65(1) |
ページ | 1-5 |
公開日 | 2019-2-8 |
DOI | 10.1262/jrd.2018-128 |
PMID | 30518723 |
PMC | PMC6379761 |
MeSH | Animals Animals, Genetically Modified / genetics CRISPR-Associated Protein 9 / genetics* CRISPR-Cas Systems / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / genetics Embryo, Mammalian* Gene Editing / methods* Mice Mice, Inbred C57BL Mutagenesis* Reproducibility of Results Whole Genome Sequencing |
IF | 1.635 |
引用数 | 6 |
リソース情報 | |
遺伝子材料 | T7-NLS hCas9-pA (RDB13130) |