RRC ID 5453
著者 Liu H, Zhou Y, Liu R, Zhang KY, Lai R.
タイトル Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel Gram-positive, motile, rod-shaped bacterium isolated from a saline soil in China was characterized by a polyphasic taxonomic approach. The strain, designated YC1(T), was halotolerant [tolerating up to 15 % (w/v) NaCl] and alkaliphilic (growing at a broad pH range of 5-13). 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Bacillus, showing highest similarity to Bacillus macauensis JCM 13285(T) (98.0 %). However, DNA-DNA hybridization indicated low levels of genomic relatedness with B. macauensis JCM 13285(T) (8.5 %). The major isoprenoid quinone was MK-7 and the cellular fatty acid profile consisted of significant amounts of iso-C(15 : 0) (38.6 %) and anteiso-C(15 : 0) (35.9 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 41.8 mol%. On the basis of the polyphasic evidence from this study, strain YC1(T) (=KCTC 13181(T)=CGMCC 1.6854(T)) should be classified as the type strain of a novel species of the genus Bacillus, for which the name Bacillus solisalsi sp. nov. is proposed.
巻・号 59(Pt 6)
ページ 1460-4
公開日 2009-6-1
DOI 10.1099/ijs.0.000653-0
PII 59/6/1460
PMID 19502335
MeSH Bacillus / classification* Bacillus / genetics Bacillus / isolation & purification Bacillus / physiology Bacterial Typing Techniques Base Composition China DNA, Bacterial / analysis DNA, Ribosomal / analysis Fatty Acids / analysis Fresh Water / microbiology* Hydrogen-Ion Concentration Lipids / analysis Molecular Sequence Data Nucleic Acid Hybridization Phenotype Phylogeny Polymerase Chain Reaction / methods RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride* / metabolism Soil Microbiology* Species Specificity
IF 2.415
引用数 17
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM13285