| RRC ID |
54592
|
| 著者 |
Takenaka Y, Kato K, Ogawa-Ohnishi M, Tsuruhama K, Kajiura H, Yagyu K, Takeda A, Takeda Y, Kunieda T, Hara-Nishimura I, Kuroha T, Nishitani K, Matsubayashi Y, Ishimizu T.
|
| タイトル |
Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family.
|
| ジャーナル |
Nat Plants
|
| Abstract |
Pectin is one of the three key cell wall polysaccharides in land plants and consists of three major structural domains: homogalacturonan, rhamnogalacturonan I (RG-I) and RG-II. Although the glycosyltransferase required for the synthesis of the homogalacturonan and RG-II backbone was identified a decade ago, those for the synthesis of the RG-I backbone, which consists of the repeating disaccharide unit [→2)-α-L-Rha-(1 → 4)-α-D-GalUA-(1→], have remained unknown. Here, we report the identification and characterization of Arabidopsis RG-I:rhamnosyltransferases (RRTs), which transfer the rhamnose residue from UDP-β-L-rhamnose to RG-I oligosaccharides. RRT1, which is one of the four Arabidopsis RRTs, is a single-spanning transmembrane protein, localized to the Golgi apparatus. RRT1 was highly expressed during formation of the seed coat mucilage, which is a specialized cell wall with abundant RG-I. Loss-of-function mutation in RRT1 caused a reduction in the level of RG-I in the seed coat mucilage. The RRTs belong to a novel glycosyltransferase family, now designated GT106. This is a large plant-specific family, and glycosyltransferases in this family seem to have plant-specific roles, such as biosynthesis of plant cell wall polysaccharides.
|
| 巻・号 |
4(9)
|
| ページ |
669-676
|
| 公開日 |
2018-9-1
|
| DOI |
10.1038/s41477-018-0217-7
|
| PII |
10.1038/s41477-018-0217-7
|
| PMID |
30082766
|
| MeSH |
Arabidopsis / enzymology*
Arabidopsis / metabolism
Arabidopsis / physiology
Arabidopsis Proteins / metabolism*
Arabidopsis Proteins / physiology
Cell Wall / metabolism
Glycosyltransferases / metabolism*
Glycosyltransferases / physiology
Pectins / metabolism*
Rhamnose / metabolism
Transcriptome
|
| IF |
13.297
|
| 引用数 |
22
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
rpc00008 |
