論文 - 詳細
| RRC ID | 54597 |
|---|---|
| 著者 | Fujimori T, Matsuda R, Suzuki M, Takenaka Y, Kajiura H, Takeda Y, Ishimizu T. |
| タイトル | Practical preparation of UDP-apiose and its applications for studying apiosyltransferase. |
| ジャーナル | Carbohydr Res |
| Abstract |
UDP-apiose, a donor substrate of apiosyltransferases, is labile because of its intramolecular self-cyclization ability, resulting in the formation of apiofuranosyl-1,2-cyclic phosphate. Therefore, stabilization of UDP-apiose is indispensable for its availability and identifying and characterizing the apiosyltransferases involved in the biosynthesis of apiosylated sugar chains and glycosides. Here, we established a method for stabilizing UDP-apiose using bulky cations as counter ions. Bulky cations such as triethylamine effectively suppressed the degradation of UDP-apiose in solution. The half-life of UDP-apiose was increased to 48.1 ± 2.4 h at pH 6.0 and 25 °C using triethylamine as a counter cation. UDP-apiose coordinated with a counter cation enabled long-term storage under freezing conditions. UDP-apiose was utilized as a donor substrate for apigenin 7-O-β-D-glucoside apiosyltransferase to produce the apiosylated glycoside apiin. This apiosyltransferase assay will be useful for identifying genes encoding apiosyltransferases. |
| 巻・号 | 477 |
| ページ | 20-25 |
| 公開日 | 2019-5-15 |
| DOI | 10.1016/j.carres.2019.03.011 |
| PII | S0008-6215(19)30056-4 |
| PMID | 30933787 |
| MeSH | Carbohydrate Conformation Enzyme Assays / methods* Pentosyltransferases / genetics Pentosyltransferases / metabolism* Uridine Diphosphate Sugars / chemical synthesis* Uridine Diphosphate Sugars / chemistry Uridine Diphosphate Sugars / metabolism* |
| IF | 1.873 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda01652 |