RRC ID 54616
著者 Nguyen NH, Cheong JJ.
タイトル AtMYB44 interacts with TOPLESS-RELATED corepressors to suppress protein phosphatase 2C gene transcription.
ジャーナル Biochem Biophys Res Commun
Abstract AtMYB44 has been described in diverse hormonal signaling processes including abscisic acid (ABA)-mediated tolerance to abiotic stress; however, its function as a transcription factor is controversial. AtMYB44 contains the amino acid sequence LSLSL, a putative ETHYLENE-RESPONSIVE ELEMENT BINDING FACTOR-ASSOCIATED AMPHIPHILIC REPRESSION (EAR) motif. In yeast two-hybrid assay, physical interaction between AtMYB44 and a TOPLESS-RELATED (TPR) corepressor was observed, but abolished by mutation of the EAR motif. We performed bimolecular fluorescence complementation assay to confirm their interaction in planta. Chromatin immunoprecipitation assay revealed binding of AtMYB44 to the promoter regions of clade A protein phosphatase 2C (PP2C) genes (e.g., ABI1, ABI2, and HAI1), implying putative targets. Levels of histone H3 acetylation around the promoter regions were markedly lower in AtMYB44-overexpressing (35S:AtMYB44) plants than in wild-type plants. These results suggest that AtMYB44 forms a complex with TPR corepressors and recruits histone deacetylase(s) to suppress PP2C gene transcription in a signal-independent manner.
巻・号 507(1-4)
ページ 437-442
公開日 2018-12-9
DOI 10.1016/j.bbrc.2018.11.057
PII S0006-291X(18)32471-9
PMID 30448055
MeSH Acetylation Arabidopsis / genetics* Arabidopsis Proteins / metabolism* Co-Repressor Proteins / metabolism* Gene Expression Regulation, Plant* Genetic Loci Histones / metabolism Plants, Genetically Modified Promoter Regions, Genetic / genetics Protein Binding Transcription Factors / metabolism* Transcription, Genetic* Two-Hybrid System Techniques
IF 2.705
引用数 4
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pdx25034 pdx79548 pda12649