Reference - Detail
| RRC ID | 54617 |
|---|---|
| Author | Kawabe H, Ohtani M, Kurata T, Sakamoto T, Demura T. |
| Title | Protein S-Nitrosylation Regulates Xylem Vessel Cell Differentiation in Arabidopsis. |
| Journal | Plant Cell Physiol |
| Abstract |
Post-translational modifications of proteins have important roles in the regulation of protein activity. One such modification, S-nitrosylation, involves the covalent binding of nitric oxide (NO)-related species to a cysteine residue. Recent work showed that protein S-nitrosylation has crucial functions in plant development and environmental responses. In the present study, we investigated the importance of protein S-nitrosylation for xylem vessel cell differentiation using a forward genetics approach. We performed ethyl methanesulfonate mutagenesis of a transgenic Arabidopsis 35S::VND7-VP16-GR line in which the activity of VASCULAR-RELATED NAC-DOMAIN7 (VND7), a key transcription factor involved in xylem vessel cell differentiation, can be induced post-translationally by glucocorticoid treatment, with the goal of obtaining suppressor mutants that failed to differentiate ectopic xylem vessel cells; we named these mutants suppressor of ectopic vessel cell differentiation induced by VND7 (seiv) mutants. We found the seiv1 mutant to be a recessive mutant in which ectopic xylem cell differentiation was inhibited, especially in aboveground organs. In seiv1 mutants, a single nucleic acid substitution (G to A) leading to an amino acid substitution (E36K) was present in the gene encoding S-NITROSOGLUTATHIONE REDUCTASE 1 (GSNOR1), which regulates the turnover of the natural NO donor, S-nitrosoglutathione. An in vitro S-nitrosylation assay revealed that VND7 can be S-nitrosylated at Cys264 and Cys320 located near the transactivation activity-related domains, which were shown to be important for transactivation activity of VND7 by transient reporter assay. Our results suggest crucial roles for GSNOR1-regulated protein S-nitrosylation in xylem vessel cell differentiation, partly through the post-translational modification of VND7. |
| Volume | 59(1) |
| Pages | 17-29 |
| Published | 2018-1-1 |
| DOI | 10.1093/pcp/pcx151 |
| PII | 4523851 |
| PMID | 29040725 |
| MeSH | Arabidopsis / cytology Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Differentiation* Cysteine / genetics Cysteine / metabolism Glutathione Reductase / genetics Glutathione Reductase / metabolism Mutation Nitric Oxide / metabolism* Plants, Genetically Modified Protein Processing, Post-Translational Transcription Factors / genetics Transcription Factors / metabolism Xylem / cytology Xylem / genetics Xylem / metabolism* |
| IF | 3.929 |
| Times Cited | 19 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | rpc00008 |