論文 - 詳細
| RRC ID | 54662 |
|---|---|
| 著者 | Castañeda Cortés DC, Padilla LFA, Langlois VS, Somoza GM, Fernandino JI. |
| タイトル | The central nervous system acts as a transducer of stress-induced masculinization through corticotropin-releasing hormone B. |
| ジャーナル | Development |
| Abstract |
Exposure to environmental stressors, such as high temperature (HT), during early development of fish induces sex reversal of genotypic females. Nevertheless, the involvement of the brain in this process is not well clarified. In the present work, we investigated the mRNA levels of corticotropin-releasing hormone b (crhb) and its receptors (crhr1 and crhr2), and found that they were upregulated at HT during the crucial period of gonadal sex determination in medaka. In order to clarify their roles in sex reversal, biallelic mutants for crhr1 and crhr2 were produced by CRISPR/Cas9 technology. Remarkably, biallelic mutants of both loci (crhr1 and crhr2) did not undergo female-to-male sex reversal upon exposure to HT. Inhibition of this process in double corticotropin-releasing hormone receptor mutants could be successfully rescued through the administration of the downstream effector of the hypothalamic-pituitary-interrenal axis, cortisol. Taken together, these results reveal for the first time that the CNS acts as a transducer of masculinization induced by thermal stress. |
| 巻・号 | 146(8) |
| 公開日 | 2019-4-23 |
| DOI | 10.1242/dev.172866 |
| PII | dev.172866 |
| PMID | 30936180 |
| MeSH | 46, XX Testicular Disorders of Sex Development / metabolism Animals Central Nervous System / metabolism* Corticotropin-Releasing Hormone / genetics Corticotropin-Releasing Hormone / metabolism* Female Genotype Hydrocortisone / metabolism Male Mutation / genetics Oryzias RNA, Messenger / genetics RNA, Messenger / metabolism |
| IF | 5.763 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 42 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | hi-medaka (MT835) |