論文 - 詳細
| RRC ID | 54670 |
|---|---|
| 著者 | Tajiri S, Yamanaka S, Fujimoto T, Matsumoto K, Taguchi A, Nishinakamura R, Okano HJ, Yokoo T. |
| タイトル | Regenerative potential of induced pluripotent stem cells derived from patients undergoing haemodialysis in kidney regeneration. |
| ジャーナル | Sci Rep |
| Abstract |
Kidney regeneration from pluripotent stem cells is receiving a lot of attention because limited treatments are currently available for chronic kidney disease (CKD). It has been shown that uremic state in CKD is toxic to somatic stem/progenitor cells, such as endothelial progenitor and mesenchymal stem cells, affecting their differentiation and angiogenic potential. Recent studies reported that specific abnormalities caused by the non-inherited disease are often retained in induced pluripotent stem cell (iPSC)-derived products obtained from patients. Thus, it is indispensable to first assess whether iPSCs derived from patients with CKD due to non-inherited disease (CKD-iPSCs) have the ability to generate kidneys. In this study, we generated iPSCs from patients undergoing haemodialysis due to diabetes nephropathy and glomerulonephritis (HD-iPSCs) as representatives of CKD-iPSCs or from healthy controls (HC-iPSCs). HD-iPSCs differentiated into nephron progenitor cells (NPCs) with similar efficiency to HC-iPSCs. Additionally, HD-iPSC-derived NPCs expressed comparable levels of NPC markers and differentiated into vascularised glomeruli upon transplantation into mice, as HC-iPSC-derived NPCs. Our results indicate the potential of HD-iPSCs as a feasible cell source for kidney regeneration. This is the first study paving the way for CKD patient-stem cell-derived kidney regeneration, emphasising the potential of CKD-iPSCs. |
| 巻・号 | 8(1) |
| ページ | 14919 |
| 公開日 | 2018-10-8 |
| DOI | 10.1038/s41598-018-33256-7 |
| PII | 10.1038/s41598-018-33256-7 |
| PMID | 30297790 |
| PMC | PMC6175865 |
| MeSH | Animals Cell Differentiation Cells, Cultured Diabetic Nephropathies / physiopathology Diabetic Nephropathies / therapy Glomerulonephritis / physiopathology Glomerulonephritis / therapy Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / transplantation Kidney / cytology* Kidney / physiology* Kidney / physiopathology Mice Nephrons / cytology Nephrons / physiology Nephrons / physiopathology Regeneration* Renal Dialysis Renal Insufficiency, Chronic / physiopathology Renal Insufficiency, Chronic / therapy* |
| IF | 4.011 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |