| RRC ID |
54670
|
| 著者 |
Tajiri S, Yamanaka S, Fujimoto T, Matsumoto K, Taguchi A, Nishinakamura R, Okano HJ, Yokoo T.
|
| タイトル |
Regenerative potential of induced pluripotent stem cells derived from patients undergoing haemodialysis in kidney regeneration.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Kidney regeneration from pluripotent stem cells is receiving a lot of attention because limited treatments are currently available for chronic kidney disease (CKD). It has been shown that uremic state in CKD is toxic to somatic stem/progenitor cells, such as endothelial progenitor and mesenchymal stem cells, affecting their differentiation and angiogenic potential. Recent studies reported that specific abnormalities caused by the non-inherited disease are often retained in induced pluripotent stem cell (iPSC)-derived products obtained from patients. Thus, it is indispensable to first assess whether iPSCs derived from patients with CKD due to non-inherited disease (CKD-iPSCs) have the ability to generate kidneys. In this study, we generated iPSCs from patients undergoing haemodialysis due to diabetes nephropathy and glomerulonephritis (HD-iPSCs) as representatives of CKD-iPSCs or from healthy controls (HC-iPSCs). HD-iPSCs differentiated into nephron progenitor cells (NPCs) with similar efficiency to HC-iPSCs. Additionally, HD-iPSC-derived NPCs expressed comparable levels of NPC markers and differentiated into vascularised glomeruli upon transplantation into mice, as HC-iPSC-derived NPCs. Our results indicate the potential of HD-iPSCs as a feasible cell source for kidney regeneration. This is the first study paving the way for CKD patient-stem cell-derived kidney regeneration, emphasising the potential of CKD-iPSCs.
|
| 巻・号 |
8(1)
|
| ページ |
14919
|
| 公開日 |
2018-10-8
|
| DOI |
10.1038/s41598-018-33256-7
|
| PII |
10.1038/s41598-018-33256-7
|
| PMID |
30297790
|
| PMC |
PMC6175865
|
| MeSH |
Animals
Cell Differentiation
Cells, Cultured
Diabetic Nephropathies / physiopathology
Diabetic Nephropathies / therapy
Glomerulonephritis / physiopathology
Glomerulonephritis / therapy
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / transplantation
Kidney / cytology*
Kidney / physiology*
Kidney / physiopathology
Mice
Nephrons / cytology
Nephrons / physiology
Nephrons / physiopathology
Regeneration*
Renal Dialysis
Renal Insufficiency, Chronic / physiopathology
Renal Insufficiency, Chronic / therapy*
|
| IF |
4.011
|
| 引用数 |
9
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063) |
