RRC ID 54670
Author Tajiri S, Yamanaka S, Fujimoto T, Matsumoto K, Taguchi A, Nishinakamura R, Okano HJ, Yokoo T.
Title Regenerative potential of induced pluripotent stem cells derived from patients undergoing haemodialysis in kidney regeneration.
Journal Sci Rep
Abstract Kidney regeneration from pluripotent stem cells is receiving a lot of attention because limited treatments are currently available for chronic kidney disease (CKD). It has been shown that uremic state in CKD is toxic to somatic stem/progenitor cells, such as endothelial progenitor and mesenchymal stem cells, affecting their differentiation and angiogenic potential. Recent studies reported that specific abnormalities caused by the non-inherited disease are often retained in induced pluripotent stem cell (iPSC)-derived products obtained from patients. Thus, it is indispensable to first assess whether iPSCs derived from patients with CKD due to non-inherited disease (CKD-iPSCs) have the ability to generate kidneys. In this study, we generated iPSCs from patients undergoing haemodialysis due to diabetes nephropathy and glomerulonephritis (HD-iPSCs) as representatives of CKD-iPSCs or from healthy controls (HC-iPSCs). HD-iPSCs differentiated into nephron progenitor cells (NPCs) with similar efficiency to HC-iPSCs. Additionally, HD-iPSC-derived NPCs expressed comparable levels of NPC markers and differentiated into vascularised glomeruli upon transplantation into mice, as HC-iPSC-derived NPCs. Our results indicate the potential of HD-iPSCs as a feasible cell source for kidney regeneration. This is the first study paving the way for CKD patient-stem cell-derived kidney regeneration, emphasising the potential of CKD-iPSCs.
Volume 8(1)
Pages 14919
Published 2018-10-8
DOI 10.1038/s41598-018-33256-7
PII 10.1038/s41598-018-33256-7
PMID 30297790
PMC PMC6175865
MeSH Animals Cell Differentiation Cells, Cultured Diabetic Nephropathies / physiopathology Diabetic Nephropathies / therapy Glomerulonephritis / physiopathology Glomerulonephritis / therapy Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / transplantation Kidney / cytology* Kidney / physiology* Kidney / physiopathology Mice Nephrons / cytology Nephrons / physiology Nephrons / physiopathology Regeneration* Renal Dialysis Renal Insufficiency, Chronic / physiopathology Renal Insufficiency, Chronic / therapy*
IF 4.011
Human and Animal Cells 201B7(HPS0063)