Reference - Detail
| RRC ID | 54671 |
|---|---|
| Author | Johzuka J, Ona T, Nomura M. |
| Title | One Hour In Vivo-like Phenotypic Screening System for Anti-cancer Drugs Using a High Precision Surface Plasmon Resonance Device. |
| Journal | Anal Sci |
| Abstract |
In anti-cancer drug (candidate) screening, there is the need for evaluation at physiological concentrations similar to in vivo. This is often performed by three-dimensionally (3D) cultured cells; however, it requires a long culture period of 2 - 4 weeks with tedious experimental procedures. Here, we report on a high precision surface plasmon resonance (HP-SPR)-3D system. We developed the system with average fluctuation of 50 ndeg s-1 using two-dimensionally cultured cells attached onto a sensor chip by applying collagen on the top to change their activity into in vivo-like conditions without cell division. It allowed in vivo-like phenotypic screening for anti-cancer drugs within 1 h of drug addition. The data were collected as the stable linear signal change parts for at least 5 min after 25 min following drug addition. The results provided compatibility to clinically related chemosensitivity test for anti-cancer (P <0.001) using two cell lines of pancreatic cancer and three anti-cancer drugs to represent differences in individual gene expression and drug mode of action. |
| Volume | 34(10) |
| Pages | 1189-1194 |
| Published | 2018-1-1 |
| DOI | 10.2116/analsci.18P013 |
| PMID | 30305596 |
| MeSH | Antineoplastic Agents / pharmacology* Cell Line, Tumor Drug Screening Assays, Antitumor / instrumentation* Humans Phenotype* Surface Plasmon Resonance / instrumentation* Time Factors |
| IF | 1.618 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | MIA Paca2(RCB2094) |