| RRC ID |
54671
|
| 著者 |
Johzuka J, Ona T, Nomura M.
|
| タイトル |
One Hour In Vivo-like Phenotypic Screening System for Anti-cancer Drugs Using a High Precision Surface Plasmon Resonance Device.
|
| ジャーナル |
Anal Sci
|
| Abstract |
In anti-cancer drug (candidate) screening, there is the need for evaluation at physiological concentrations similar to in vivo. This is often performed by three-dimensionally (3D) cultured cells; however, it requires a long culture period of 2 - 4 weeks with tedious experimental procedures. Here, we report on a high precision surface plasmon resonance (HP-SPR)-3D system. We developed the system with average fluctuation of 50 ndeg s-1 using two-dimensionally cultured cells attached onto a sensor chip by applying collagen on the top to change their activity into in vivo-like conditions without cell division. It allowed in vivo-like phenotypic screening for anti-cancer drugs within 1 h of drug addition. The data were collected as the stable linear signal change parts for at least 5 min after 25 min following drug addition. The results provided compatibility to clinically related chemosensitivity test for anti-cancer (P <0.001) using two cell lines of pancreatic cancer and three anti-cancer drugs to represent differences in individual gene expression and drug mode of action.
|
| 巻・号 |
34(10)
|
| ページ |
1189-1194
|
| 公開日 |
2018-1-1
|
| DOI |
10.2116/analsci.18P013
|
| PMID |
30305596
|
| MeSH |
Antineoplastic Agents / pharmacology*
Cell Line, Tumor
Drug Screening Assays, Antitumor / instrumentation*
Humans
Phenotype*
Surface Plasmon Resonance / instrumentation*
Time Factors
|
| IF |
1.618
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
MIA Paca2(RCB2094) |
