| RRC ID |
54689
|
| 著者 |
Sato M, Asano T, Hosomichi J, Ono T, Nakata T.
|
| タイトル |
Optogenetic manipulation of intracellular calcium by BACCS promotes differentiation of MC3T3-E1 cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Bone remodeling is maintained through the balance between bone formation by osteoblasts and bone resorption by osteoclasts. Previous studies suggested that intracellular Ca2+ signaling plays an important role in the differentiation of osteoblasts; however, the molecular mechanism of Ca2+ signaling in the differentiation of osteoblasts remains unclear. To elucidate the effect of Ca2+ signaling in osteoblasts, we employed an optogenetic tool, blue light-activated Ca2+ channel switch (BACCS). BACCS was used to spatiotemporally control intracellular Ca2+ with blue light stimulation. MC3T3-E1 cells, which have been used as a model of differentiation from preosteoblast to osteoblast, were promoted to differentiate by BACCS expression and rhythmical blue light stimulation. The results indicated that intracellular Ca2+ change from the outside of the cells can regulate signaling for differentiation of MC3T3-E1 cells. Our findings provide evidence that Ca2+ could cause osteoblast differentiation.
|
| 巻・号 |
506(3)
|
| ページ |
716-722
|
| 公開日 |
2018-11-30
|
| DOI |
10.1016/j.bbrc.2018.10.107
|
| PII |
S0006-291X(18)32269-1
|
| PMID |
30376992
|
| MeSH |
Animals
Calcium / metabolism*
Calcium Channels / metabolism*
Calcium Signaling
Cell Differentiation*
Cell Line
Intracellular Space / metabolism
Ion Channel Gating* / radiation effects
Light*
Mice, Inbred C57BL
Optogenetics*
Osteoclasts / cytology
Osteoclasts / metabolism
|
| IF |
2.705
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
