RRC ID 547
Author Seki Y, Aonuma H, Kanzaki R.
Title Pheromone processing center in the protocerebrum of Bombyx mori revealed by nitric oxide-induced anti-cGMP immunocytochemistry.
Journal J Comp Neurol
Abstract The antennal lobe (AL) of the male silkworm moth Bombyx mori contains 60 +/- 2 ventrally located antennal glomeruli and a dorsal macroglomerular complex (MGC) consisting of three subdivisions. The response patterns of MGC projection neurons (PNs) to pheromonal stimuli correlate with their dendritic arborization in the subdivisions of the MGC. However, the representation of this pheromonal information in the lateral protocerebrum (LPC), which is the target site of the AL PNs, is not well known. We performed nitric oxide (NO)-induced anti-cGMP immunohistochemistry and found that the PNs which respond to the major pheromone component (bombykol) express strong immunoreactivity. They project to a specific area, the delta area in the inferior lateral protocerebrum (DeltaILPC), which clearly represents the processing center for the major pheromone component. Furthermore, to examine the projection sites in the LPC from each subdivision of the MGC, we performed double-labeling of each type of MGC-PNs, combined with NO-induced anti-cGMP immunohistochemistry. We revealed that projections from each subdivision of the MGC overlapped or separated in specific regions of the DeltaILPC. These results suggest that integration and segregation of pheromone information may occur in the DeltaILPC.
Volume 481(4)
Pages 340-51
Published 2005-1-24
DOI 10.1002/cne.20392
PMID 15593336
MeSH Animals Bombyx / anatomy & histology Bombyx / physiology* Brain / cytology Brain / physiology* Brain Mapping* Cyclic GMP / metabolism Dendrites / metabolism Imaging, Three-Dimensional Immunohistochemistry Male Neurons, Afferent / physiology Nitric Oxide / metabolism Olfactory Pathways / cytology Olfactory Pathways / physiology* Pheromones / physiology* Signal Transduction / physiology Smell / physiology*
IF 2.801
Times Cited 64