RRC ID 54706
著者 Nakanishi J, Sugiyama K, Matsuo H, Takahashi Y, Omura S, Nakashima T.
タイトル An Application of Photoactivatable Substrate for the Evaluation of Epithelial-mesenchymal Transition Inhibitors.
ジャーナル Anal Sci
Abstract Epithelial-mesenchymal transition (EMT), phenotypic changes in cell adhesion and migration, is involved in cancer invasion and metastasis, hence becoming a target for anti-cancer drugs. In this study, we report a method for the evaluation of EMT inhibitors by using a photoactivatable gold substrate, which changes from non-cell-adhesive to cell-adhesive in response to light. The method is based on the geometrical confinement of cell clusters and the subsequent migration induction by controlled photoirradiation of the substrate. As a proof-of-concept experiment, a known EMT inhibitor was successfully evaluated in terms of the changes in cluster area or leader cell appearance, in response to biochemically and mechanically induced EMT. Furthermore, an application of the present method for microbial secondary metabolites identified nanaomycin H as an EMT inhibitor, potentially killing EMTed cells in disseminated conditions. These results demonstrate the potential of the present method for screening new EMT inhibitors.
巻・号 35(1)
ページ 65-69
公開日 2019-1-10
DOI 10.2116/analsci.18SDP07
PMID 30393243
MeSH Animals Antineoplastic Agents / pharmacology Cell Adhesion / drug effects Cell Movement / drug effects Cell Survival / drug effects Dogs Drug Evaluation, Preclinical / methods* Epithelial-Mesenchymal Transition / drug effects* Gold / chemistry* Madin Darby Canine Kidney Cells Nitrobenzenes / chemistry* Nitrobenzenes / radiation effects Polyethylene Glycols / chemistry* Proof of Concept Study Ultraviolet Rays*
IF 1.618
引用数 2
リソース情報
ヒト・動物細胞 MDCK(RCB0995)