RRC ID |
5472
|
著者 |
Shiratori H, Sasaya K, Ohiwa H, Ikeno H, Ayame S, Kataoka N, Miya A, Beppu T, Ueda K.
|
タイトル |
Clostridium clariflavum sp. nov. and Clostridium caenicola sp. nov., moderately thermophilic, cellulose-/cellobiose-digesting bacteria isolated from methanogenic sludge.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
Two novel anaerobic, moderately thermophilic and cellulose-/cellobiose-digesting bacteria, EBR45(T) and EBR596(T), were isolated from anaerobic sludge of a cellulose-degrading methanogenic bioreactor. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belonged to cluster III within the low-G+C-content Gram-positive bacteria. The close relatives of EBR45(T) were Clostridium straminisolvens DSM 16021(T) (sequence identity, 94.6 %) and Clostridium thermocellum DSM 1237(T) (93.4 %). The closest relative of EBR596(T) was Clostridium stercorarium DSM 8532(T) (95.9 %). Both isolates were rod-shaped sporulators, growing optimally at 60 degrees C. EBR45(T) was Gram-staining-reaction-variable and non-motile, formed bright-yellow colonies on solid media, and grew on a relatively narrow range of carbohydrates including cellulose and cellobiose. EBR596(T) was Gram-staining-reaction-negative and motile, formed glossy white colonies and grew on cellobiose and various carbohydrates except cellulose. Major fatty acid compositions were 16 : 0 iso, 16 : 0 and 16 : 0 dimethylacetal (strain EBR45(T)) and 15 : 0 iso, 16 : 0 iso, 15 : 0 anteiso and 17 : 0 anteiso (strain EBR596(T)). The DNA G+C contents were 36.9 mol% (EBR45(T)) and 51.1 mol% (EBR596(T)). Based on the phenotypic and phylogenetic data and genomic distinctiveness, strains EBR45(T) and EBR596(T) represent two novel species, for which the names Clostridium clariflavum sp. nov. (type strain EBR45(T) =DSM 19732(T) =NBRC 101661(T)) and Clostridium caenicola sp. nov. (type strain EBR596(T) =DSM 19027(T) =NBRC 102590(T)) are proposed.
|
巻・号 |
59(Pt 7)
|
ページ |
1764-70
|
公開日 |
2009-7-1
|
DOI |
10.1099/ijs.0.003483-0
|
PII |
ijs.0.003483-0
|
PMID |
19542130
|
MeSH |
Bacterial Typing Techniques
Base Composition
Cellobiose / metabolism*
Cellulose / metabolism*
Clostridium / classification*
Clostridium / genetics
Clostridium / isolation & purification
Clostridium / physiology
DNA, Bacterial / analysis
Fatty Acids / analysis
Genes, rRNA
Genotype
Hot Temperature*
Methane / metabolism*
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sewage / microbiology*
Species Specificity
|
IF |
2.415
|
引用数 |
71
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM21531 |