RRC ID 5472
著者 Shiratori H, Sasaya K, Ohiwa H, Ikeno H, Ayame S, Kataoka N, Miya A, Beppu T, Ueda K.
タイトル Clostridium clariflavum sp. nov. and Clostridium caenicola sp. nov., moderately thermophilic, cellulose-/cellobiose-digesting bacteria isolated from methanogenic sludge.
ジャーナル Int J Syst Evol Microbiol
Abstract Two novel anaerobic, moderately thermophilic and cellulose-/cellobiose-digesting bacteria, EBR45(T) and EBR596(T), were isolated from anaerobic sludge of a cellulose-degrading methanogenic bioreactor. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belonged to cluster III within the low-G+C-content Gram-positive bacteria. The close relatives of EBR45(T) were Clostridium straminisolvens DSM 16021(T) (sequence identity, 94.6 %) and Clostridium thermocellum DSM 1237(T) (93.4 %). The closest relative of EBR596(T) was Clostridium stercorarium DSM 8532(T) (95.9 %). Both isolates were rod-shaped sporulators, growing optimally at 60 degrees C. EBR45(T) was Gram-staining-reaction-variable and non-motile, formed bright-yellow colonies on solid media, and grew on a relatively narrow range of carbohydrates including cellulose and cellobiose. EBR596(T) was Gram-staining-reaction-negative and motile, formed glossy white colonies and grew on cellobiose and various carbohydrates except cellulose. Major fatty acid compositions were 16 : 0 iso, 16 : 0 and 16 : 0 dimethylacetal (strain EBR45(T)) and 15 : 0 iso, 16 : 0 iso, 15 : 0 anteiso and 17 : 0 anteiso (strain EBR596(T)). The DNA G+C contents were 36.9 mol% (EBR45(T)) and 51.1 mol% (EBR596(T)). Based on the phenotypic and phylogenetic data and genomic distinctiveness, strains EBR45(T) and EBR596(T) represent two novel species, for which the names Clostridium clariflavum sp. nov. (type strain EBR45(T) =DSM 19732(T) =NBRC 101661(T)) and Clostridium caenicola sp. nov. (type strain EBR596(T) =DSM 19027(T) =NBRC 102590(T)) are proposed.
巻・号 59(Pt 7)
ページ 1764-70
公開日 2009-7-1
DOI 10.1099/ijs.0.003483-0
PII ijs.0.003483-0
PMID 19542130
MeSH Bacterial Typing Techniques Base Composition Cellobiose / metabolism* Cellulose / metabolism* Clostridium / classification* Clostridium / genetics Clostridium / isolation & purification Clostridium / physiology DNA, Bacterial / analysis Fatty Acids / analysis Genes, rRNA Genotype Hot Temperature* Methane / metabolism* Molecular Sequence Data Nucleic Acid Hybridization Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sewage / microbiology* Species Specificity
IF 2.415
引用数 71
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM21531