論文 - 詳細
| RRC ID | 54744 |
|---|---|
| 著者 | Kamiki H, Matsugo H, Kobayashi T, Ishida H, Takenaka-Uema A, Murakami S, Horimoto T. |
| タイトル | A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice. |
| ジャーナル | Viruses |
| Abstract |
H9N2 avian influenza viruses are present in poultry worldwide. These viruses are considered to have pandemic potential, because recent isolates can recognize human-type receptor and several sporadic human infections have been reported. In this study, we aimed to identify mutations related to mammalian adaptation of H9N2 influenza virus. We found that mouse-adapted viruses had several mutations in hemagglutinin (HA), PB2, PA, and PB1. Among the detected mutations, PB1-K577E was a novel mutation that had not been previously reported to involve mammalian adaptation. A recombinant H9N2 virus bearing only the PB1-K577E mutation showed enhanced pathogenicity in mice, with increased virus titers in nasal turbinates compared to that in mice infected with the wild-type virus. In addition, the PB1-K577E mutation increased virus polymerase activity in human cell culture at a lower temperature. These data suggest that the PB1-K577E mutation is a novel pathogenicity determinant of H9N2 virus in mice and could be a signature for mammalian adaptation. |
| 巻・号 | 10(11) |
| 公開日 | 2018-11-19 |
| DOI | 10.3390/v10110653 |
| PII | v10110653 |
| PMID | 30463209 |
| PMC | PMC6266086 |
| MeSH | Adaptation, Biological Animals Disease Models, Animal HEK293 Cells Humans Influenza A Virus, H9N2 Subtype / enzymology* Influenza A Virus, H9N2 Subtype / genetics Influenza A Virus, H9N2 Subtype / pathogenicity* Mice Mutation, Missense* Nasal Cavity / virology Orthomyxoviridae Infections / pathology Orthomyxoviridae Infections / virology* Temperature Viral Load Viral Proteins / genetics* Viral Proteins / metabolism* Virus Replication / radiation effects |
| IF | 3.811 |
| 引用数 | 11 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |