| RRC ID |
54745
|
| Author |
Imamura T, Isozumi N, Higashimura Y, Miyazato A, Mizukoshi H, Ohki S, Mori M.
|
| Title |
Isolation of amaranthin synthetase from Chenopodium quinoa and construction of an amaranthin production system using suspension-cultured tobacco BY-2 cells.
|
| Journal |
Plant Biotechnol J
|
| Abstract |
Betalains are plant pigments primarily produced by plants of the order Caryophyllales. Because betalain possesses anti-inflammatory and anticancer activities, it may be useful as a pharmaceutical agent and dietary supplement. Recent studies have identified the genes involved in the betalain biosynthesis of betanin. Amaranthin and celosianin II are abundant in the quinoa (Chenopodium quinoa Willd.) hypocotyl, and amaranthin comprises glucuronic acid bound to betanin; therefore, this suggests the existence of a glucuronyltransferase involved in the synthesis of amaranthin in the quinoa hypocotyl. To identify the gene involved in amaranthin biosynthesis, we performed a BLAST analysis and phylogenetic tree analysis based on sequences homologous to flavonoid glycosyltransferase, followed by expression analysis on the quinoa hypocotyl to obtain three candidate proteins. Production of amaranthin in a transient Nicotiana benthamiana expression system was evaluated for these candidates and one was identified as having the ability to produce amaranthin. The gene encoding this protein was quinoa amaranthin synthetase 1 (CqAmaSy1). We also created a transgenic tobacco bright yellow-2 (BY-2) cell line wherein four betalain biosynthesis genes were introduced to facilitate amaranthin production. This transgenic cell line produced 13.67 ± 4.13 μm (mean ± SEM) amaranthin and 26.60 ± 1.53 μm betanin, whereas the production of isoamaranthin and isobetanin could not be detected. Tests confirmed the ability of amaranthin and betanin to slightly suppress cancer cell viability. Furthermore, amaranthin was shown to significantly inhibit HIV-1 protease activity, whereas betanin did not.
|
| Volume |
17(5)
|
| Pages |
969-981
|
| Published |
2019-5-1
|
| DOI |
10.1111/pbi.13032
|
| PMID |
30451369
|
| PMC |
PMC6587806
|
| MeSH |
Betacyanins / biosynthesis*
Betacyanins / metabolism
Bioreactors
Cells, Cultured
Chenopodium quinoa / enzymology*
Chenopodium quinoa / metabolism
Cloning, Molecular
HIV Protease
HIV Protease Inhibitors / metabolism
HIV Protease Inhibitors / pharmacology
Ligases / isolation & purification*
Ligases / metabolism
Metabolic Networks and Pathways
Plant Proteins / isolation & purification*
Plant Proteins / metabolism
Plants, Genetically Modified
Reverse Transcriptase Polymerase Chain Reaction
Tobacco / cytology
Tobacco / enzymology
Tobacco / metabolism*
|
| IF |
6.84
|
| Times Cited |
0
|
| Resource |
| Human and Animal Cells |
MCF7(RCB1904) |
| Arabidopsis / Cultured plant cells, genes |
rpc00001 |
