論文 - 詳細
| RRC ID | 54763 |
|---|---|
| 著者 | Liu G, Wang Y, Zhang M, Zhang Q. |
| タイトル | Long non-coding RNA THRIL promotes LPS-induced inflammatory injury by down-regulating microRNA-125b in ATDC5 cells. |
| ジャーナル | Int Immunopharmacol |
| Abstract |
BACKGROUND:Osteoarthritis is an age-related disorder of bone-joint that causes pain and disability in middle and older people. This study aimed to investigate the potential effects of long non-coding RNA (lncRNA) THRIL on lipopolysaccharide (LPS)-induced osteoarthritis cell injury model (ATDC5 cell inflammatory injury), as well as the possible internal molecular mechanisms. METHODS:Cell viability and apoptosis were assessed using CCK-8 assay and Guava Nexin assay, respectively. Cell transfection was conducted to change the expression of THRIL and microRNA-125b (miR-125b) in ATDC5 cells. qRT-PCR was performed to detect the expression of THRIL, miR-125b and pro-inflammatory cytokines IL-6, TNF-α and monocyte chemotactic protein 1 (MCP-1) in ATDC5 cells. ELISA was used to measure the concentrations of IL-6, TNF-α and MCP-1 in culture supernatant of ATDC5 cells. Finally, the protein expression of key factors involved in cell apoptosis, inflammatory response, JAK1/STAT3 and NF-κB pathways were evaluated using western blotting. RESULTS:LPS significantly induced ATDC5 cell inflammatory injury and up-regulated the expression of THRIL. Overexpression of THRIL aggravated the LPS-induced ATDC5 cell inflammatory injury. Suppression of THRIL had opposite effects. Moreover, THRIL negatively regulated the expression of miR-125b in ATDC5 cells. miR-125b participated in the effects of THRIL overexpression on LPS-induced ATDC5 cell inflammatory injury. Furthermore, overexpression of THRIL enhanced the LPS-induced JAK1/STAT3 and NF-κB pathways activation by down-regulating miR-125b. CONCLUSION:THRIL exerted pro-inflammatory roles in LPS-induced osteoarthritis cell injury model. Overexpression of THRIL promoted LPS-induced ATDC5 cell inflammatory injury by down-regulating miR-125b and then activating JAK1/STAT3 and NF-κB pathways. |
| 巻・号 | 66 |
| ページ | 354-361 |
| 公開日 | 2019-1-1 |
| DOI | 10.1016/j.intimp.2018.11.038 |
| PII | S1567-5769(18)31226-8 |
| PMID | 30521964 |
| MeSH | Apoptosis Cell Line Cell Survival Chondrocytes / physiology* Down-Regulation Humans Inflammation / genetics* Janus Kinase 1 / metabolism Lipopolysaccharides / immunology MicroRNAs / genetics* NF-kappa B / metabolism Osteoarthritis / genetics* RNA, Long Noncoding / genetics* Signal Transduction Tumor Necrosis Factor-alpha / metabolism |
| IF | 3.361 |
| 引用数 | 10 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |