論文 - 詳細
| RRC ID | 54800 |
|---|---|
| 著者 | Hatano H, Goda T, Matsumoto A, Miyahara Y. |
| タイトル | Induced Proton Perturbation for Sensitive and Selective Detection of Tight Junction Breakdown. |
| ジャーナル | Anal Chem |
| Abstract |
Tight junctions (TJs) in the epithelial cell gap play primary roles in body defense and nutrient absorption in multicellular organisms. Standard in vitro assays lack sensitivity, selectivity, temporal resolution, and throughput for bioengineering applications. Prompted by the rigorous barrier functions of TJ, we developed a TJ assay that senses proton leaks in the cell gap using ion-sensitive field-effect transistors. Upon exposure of Madin-Darby canine kidney (MDCK) cells cultured on a gate dielectric to a calcium-chelator EGTA, ammonia-assisted pH perturbation was enhanced solely in TJ-forming cells. This was supported by simulations with increased ion permeability in the paracellular pathway. Following administration of Clostridium perfringens enterotoxin as a specific TJ inhibitor to the MDCK cells, our method detected TJ breakdown at a 13× lower concentration than a conventional trans-epithelial electrical resistance assay. Thus, the semiconductor-based active pH sensing could offer an alternative to current in vitro assays for TJs in pathological, toxicological, and pharmaceutical studies. |
| 巻・号 | 91(5) |
| ページ | 3525-3532 |
| 公開日 | 2019-3-5 |
| DOI | 10.1021/acs.analchem.8b05237 |
| PMID | 30596415 |
| MeSH | Animals Bioengineering Cells, Cultured Clostridium perfringens / chemistry Dogs Enterotoxins / administration & dosage Enterotoxins / pharmacology Hydrogen-Ion Concentration Madin Darby Canine Kidney Cells / drug effects Madin Darby Canine Kidney Cells / metabolism Protons* Semiconductors Tight Junctions / drug effects Tight Junctions / metabolism* |
| IF | 6.35 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) NIH/3T3(RCB2767) |