| RRC ID |
54809
|
| 著者 |
Honda-Uezono A, Kaida A, Michi Y, Harada K, Hayashi Y, Hayashi Y, Miura M.
|
| タイトル |
Unusual expression of red fluorescence at M phase induced by anti-microtubule agents in HeLa cells expressing the fluorescent ubiquitination-based cell cycle indicator (Fucci).
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Plinabulin (NPI-2358) is a novel microtubule-depolymerizing agent. In HeLa cells, plinabulin arrests the cell-cycle at M phase and subsequently induces mitotic catastrophe. To better understand the effects on this compound on the cell-cycle, we used the fluorescent ubiquitination-based cell cycle indicator (Fucci), which normally enables G1 and S/G2/M cells to emit red and green fluorescence, respectively. When HeLa-Fucci cells were treated with 50 nM plinabulin, cells began to fluoresce both green and red in an unusual pattern; most cells exhibited the new pattern after 24 h of treatment. X-irradiation efficiently induced G2 arrest in plinabulin-treated cells and significantly retarded the emergence of the unusual pattern, suggesting that entering M phase is essential for induction of the pattern. By simultaneously visualizing chromosomes with GFP-histone H2B, we established that the pattern emerges after nuclear envelope breakdown but before metaphase. Pedigree assay revealed a significant relationship between the unusual expression and mitotic catastrophe. Nocodazole, KPU-133 (a more potent derivative of plinabulin), and paclitaxel also exerted similar effects. From these data, we conclude that the unusual pattern may be associated with dysregulation of late M phase-specific E3 ligase activity and mitotic catastrophe following treatment with anti-microtubule agents.
|
| 巻・号 |
428(2)
|
| ページ |
224-9
|
| 公開日 |
2012-11-16
|
| DOI |
10.1016/j.bbrc.2012.10.014
|
| PII |
S0006-291X(12)01938-9
|
| PMID |
23063846
|
| MeSH |
Cell Division / drug effects*
Diketopiperazines / pharmacology*
Fluorescence*
HeLa Cells
Humans
Luminescent Proteins / analysis
Luminescent Proteins / biosynthesis*
Microtubules / drug effects*
Mitosis / drug effects*
Nocodazole / pharmacology
Tubulin Modulators / pharmacology*
Ubiquitin-Protein Ligases / metabolism
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa |
