RRC ID 54850
Author Ishibashi Y, Nakamura O, Yamagami Y, Nishimura H, Fukuoka N, Yamamoto T.
Title Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells.
Journal Anticancer Res.
Abstract BACKGROUND/AIM:We previously showed that the use of autophagy inhibitors in combination with chemotherapy can enhance anticancer effects in sarcoma cell lines. In this study, we investigated the combined effect of the autophagy inhibitor chloroquine and the mTOR inhibitor rapamycin on MG63 osteosarcoma cells.
MATERIALS AND METHODS:Effects of chloroquine and/or rapamycin on cell proliferation were assessed by WST-1 assays. Effects of chloroquine and/or rapamycin on the mTOR pathway components, autophagy, and apoptosis were investigated by western blot, flow cytometry, and fluorescence microscopy using immunocytochemical staining of LC3 and Annexin V-FITC/propidium iodide.
RESULTS:Rapamycin suppressed cell growth and inhibited the mTOR pathway. Rapamycin promoted autophagy by blocking the mTOR pathway, and chloroquine enhanced apoptosis by blocking autophagy.
CONCLUSION:Chloroquine enhances the effects of rapamycin in inducing apoptosis via autophagy inhibition in MG63 cells. Thus, the combined therapy of chloroquine and rapamycin may be a potent treatment for osteosarcoma.
Volume 39(2)
Pages 649-654
Published 2019-2
DOI 10.21873/anticanres.13159
PII 39/2/649
PMID 30711941
MeSH Antibiotics, Antineoplastic / pharmacology Apoptosis / drug effects* Autophagy* Bone Neoplasms / pathology* Cell Line, Tumor Cell Proliferation Chloroquine / pharmacology* Dose-Response Relationship, Drug Drug Synergism Humans Microscopy, Fluorescence Osteosarcoma / pathology* Sirolimus / pharmacology* TOR Serine-Threonine Kinases / metabolism
IF 1.935
Human and Animal Cells MG-63(RCB1890)