Reference - Detail
| RRC ID | 54852 |
|---|---|
| Author | Shinada H, Watanabe T, Okudaira K, Iwase Y, Nishi K, Yumita N. |
| Title | Apoptosis Induced by Ultraviolet A Exposure in the Presence of Enoxacin in HL-60 Cells. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND:The ultraviolet A (UVA) spectrum mainly includes the region associated with the phototoxicity of fluoroquinolone antimicrobial agents. This study investigated apoptosis induced with UVA light and enoxacin in HL-60 cells. MATERIALS AND METHODS:HL-60 cells were irradiated by UVA (1.1 mW/cm2) for 20 min in the presence or absence of enoxacin. The induction of apoptosis was investigated by analysing cell morphology, flow cytometry of annexin V-positive cells, DNA ladder formation, and caspase-3 activation. RESULTS:Significant induction of apoptosis, DNA fragmentation, and caspase-3 activation were observed in cells treated with both UVA and enoxacin. UVA-induced apoptosis was significantly suppressed when NaN3, a singlet oxygen scavenger, was present. CONCLUSION:Apoptosis was induced by the combination of UVA and enoxacin in HL-60 cells, and singlet oxygen appears to play an important role in photodynamically-induced apoptosis. |
| Volume | 39(2) |
| Pages | 687-693 |
| Published | 2019-2-1 |
| DOI | 10.21873/anticanres.13164 |
| PII | 39/2/687 |
| PMID | 30711946 |
| MeSH | Apoptosis* Caspase 3 / metabolism DNA / drug effects* DNA / radiation effects* DNA Fragmentation / drug effects Enoxacin / pharmacology* Flow Cytometry Fluoroquinolones / pharmacology Free Radical Scavengers HL-60 Cells Humans Photosensitizing Agents / chemistry Reactive Oxygen Species Sodium Azide / chemistry Ultraviolet Rays* |
| IF | 1.935 |
| Times Cited | 1 |
| Resource | |
| Human and Animal Cells | HL-60(RCB3683) |