Reference - Detail
| RRC ID | 54865 |
|---|---|
| Author | Kang JH, Miettinen TP, Chen L, Olcum S, Katsikis G, Doyle PS, Manalis SR. |
| Title | Noninvasive monitoring of single-cell mechanics by acoustic scattering. |
| Journal | Nat Methods |
| Abstract |
The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized acoustic scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that acoustic scattering can be used to noninvasively probe subtle and transient dynamics. |
| Volume | 16(3) |
| Pages | 263-269 |
| Published | 2019-3-1 |
| DOI | 10.1038/s41592-019-0326-x |
| PII | 10.1038/s41592-019-0326-x |
| PMID | 30742041 |
| PMC | PMC6420125 |
| MeSH | Acoustics* Animals Biomechanical Phenomena Cell Cycle Dactinomycin / metabolism Mice Microfluidics Single-Cell Analysis / methods* |
| IF | 28.467 |
| Times Cited | 9 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 30 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Ba/F3(RCB0805) |