| RRC ID |
54905
|
| 著者 |
Mori H, Tomiyasu T, Nishiyama K, Matsumoto M, Osawa Y, Okazaki K.
|
| タイトル |
L233P mutation in the bovine leukemia virus Tax protein depresses endothelial cell recruitment and tumorigenesis in athymic nude mice.
|
| ジャーナル |
Arch Virol
|
| Abstract |
Bovine leukemia virus (BLV) can be divided into two categories based on the amino acid at position 233 in the Tax protein, which probably plays a crucial role in leukemogenesis. We show here that a rat fibroblast cell line stably expressing L233-Tax formed significantly larger tumors than P233-Tax-expressing cells in a murine xenograft study. Although the microvessel density was comparable in both tumors, visible blood vessel invasion was observed only on tumors from L233-Tax-expressing cells. Endothelial cell tube formation assays using human umbilical vein endothelial cells showed no significant difference in angiogenic activity between conditioned medium from L233- and P233-Tax-expressing cells, whereas in vitro chemotaxis assays revealed that only L233-Tax-expressing cells produced a chemoattractant for endothelial cells. Since pathological neovascularization can occur from the recruitment of endothelial progenitor cells, these results suggest that L233-Tax-expressing cells recruit murine endothelial progenitor cells and promote neovascularization to support tumor growth. BLV-infected lymphoma cells may also recruit bovine endothelial progenitor cells to promote neovascularization. The findings of this study are consistent with our previous observation that BLV carrying P233-Tax has a significantly longer incubation period for developing tumors than the virus carrying L233-Tax and provide insight into the function of Tax in leukemogenesis by BLV.
|
| 巻・号 |
164(5)
|
| ページ |
1343-1351
|
| 公開日 |
2019-5-1
|
| DOI |
10.1007/s00705-019-04191-3
|
| PII |
10.1007/s00705-019-04191-3
|
| PMID |
30848389
|
| MeSH |
Animals
Carcinogenesis / genetics*
Cattle
Cell Line
Chemotaxis / physiology
Endothelial Cells / physiology*
Enzootic Bovine Leukosis / pathology*
Female
Gene Products, tax / genetics*
Human Umbilical Vein Endothelial Cells
Humans
Leukemia Virus, Bovine / genetics*
Leukemia Virus, Bovine / pathogenicity
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Neovascularization, Pathologic / genetics*
Neovascularization, Pathologic / pathology
Plasmids / genetics
Rats
Transplantation, Heterologous
|
| IF |
2.261
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
Rat-1(RCB1830) |
