論文 - 詳細
| RRC ID | 54905 |
|---|---|
| 著者 | Mori H, Tomiyasu T, Nishiyama K, Matsumoto M, Osawa Y, Okazaki K. |
| タイトル | L233P mutation in the bovine leukemia virus Tax protein depresses endothelial cell recruitment and tumorigenesis in athymic nude mice. |
| ジャーナル | Arch Virol |
| Abstract |
Bovine leukemia virus (BLV) can be divided into two categories based on the amino acid at position 233 in the Tax protein, which probably plays a crucial role in leukemogenesis. We show here that a rat fibroblast cell line stably expressing L233-Tax formed significantly larger tumors than P233-Tax-expressing cells in a murine xenograft study. Although the microvessel density was comparable in both tumors, visible blood vessel invasion was observed only on tumors from L233-Tax-expressing cells. Endothelial cell tube formation assays using human umbilical vein endothelial cells showed no significant difference in angiogenic activity between conditioned medium from L233- and P233-Tax-expressing cells, whereas in vitro chemotaxis assays revealed that only L233-Tax-expressing cells produced a chemoattractant for endothelial cells. Since pathological neovascularization can occur from the recruitment of endothelial progenitor cells, these results suggest that L233-Tax-expressing cells recruit murine endothelial progenitor cells and promote neovascularization to support tumor growth. BLV-infected lymphoma cells may also recruit bovine endothelial progenitor cells to promote neovascularization. The findings of this study are consistent with our previous observation that BLV carrying P233-Tax has a significantly longer incubation period for developing tumors than the virus carrying L233-Tax and provide insight into the function of Tax in leukemogenesis by BLV. |
| 巻・号 | 164(5) |
| ページ | 1343-1351 |
| 公開日 | 2019-5-1 |
| DOI | 10.1007/s00705-019-04191-3 |
| PII | 10.1007/s00705-019-04191-3 |
| PMID | 30848389 |
| MeSH | Animals Carcinogenesis / genetics* Cattle Cell Line Chemotaxis / physiology Endothelial Cells / physiology* Enzootic Bovine Leukosis / pathology* Female Gene Products, tax / genetics* Human Umbilical Vein Endothelial Cells Humans Leukemia Virus, Bovine / genetics* Leukemia Virus, Bovine / pathogenicity Mice Mice, Inbred BALB C Mice, Nude Neoplasm Transplantation Neovascularization, Pathologic / genetics* Neovascularization, Pathologic / pathology Plasmids / genetics Rats Transplantation, Heterologous |
| IF | 2.261 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Rat-1(RCB1830) |