| RRC ID |
54908
|
| 著者 |
Seah MKY, Wang Y, Goy PA, Loh HM, Peh WJ, Low DHP, Han BY, Wong E, Leong EL, Wolf G, Mzoughi S, Wollmann H, Macfarlan TS, Guccione E, Messerschmidt DM.
|
| タイトル |
The KRAB-zinc-finger protein ZFP708 mediates epigenetic repression at RMER19B retrotransposons.
|
| ジャーナル |
Development
|
| Abstract |
Global epigenetic reprogramming is vital to purge germ cell-specific epigenetic features to establish the totipotent state of the embryo. This process transpires to be carefully regulated and is not an undirected, radical erasure of parental epigenomes. The TRIM28 complex has been shown to be crucial in embryonic epigenetic reprogramming by regionally opposing DNA demethylation to preserve vital parental information to be inherited from germline to soma. Yet the DNA-binding factors guiding this complex to specific targets are largely unknown. Here, we uncover and characterize a novel, maternally expressed, TRIM28-interacting KRAB zinc-finger protein: ZFP708. It recruits the repressive TRIM28 complex to RMER19B retrotransposons to evoke regional heterochromatin formation. ZFP708 binding to these hitherto unknown TRIM28 targets is DNA methylation and H3K9me3 independent. ZFP708 mutant mice are viable and fertile, yet embryos fail to inherit and maintain DNA methylation at ZFP708 target sites. This can result in activation of RMER19B-adjacent genes, while ectopic expression of ZFP708 results in transcriptional repression. Finally, we describe the evolutionary conservation of ZFP708 in mice and rats, which is linked to the conserved presence of the targeted RMER19B retrotransposons in these species.
|
| 巻・号 |
146(19)
|
| 公開日 |
2019-7-10
|
| DOI |
10.1242/dev.170266
|
| PII |
dev.170266
|
| PMID |
30846446
|
| PMC |
PMC6803371
|
| MeSH |
Animals
Base Sequence
Binding Sites / genetics
Blastocyst / metabolism
DNA Methylation / genetics
Embryo, Mammalian / metabolism
Epigenetic Repression*
Evolution, Molecular
Mice
Mice, Knockout
Mouse Embryonic Stem Cells / metabolism
Protein Binding / genetics
Rats
Repressor Proteins / metabolism*
Retroelements / genetics*
Transcription, Genetic
Tripartite Motif-Containing Protein 28 / metabolism
Zinc Fingers*
|
| IF |
5.763
|
| 引用数 |
0
|
| リソース情報 |
| ヒト・動物細胞 |
Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19)(AES0146) |
