RRC ID 54916
著者 Matsumoto E, Koide N, Hanzawa H, Kiyama M, Ohta M, Kuwabara J, Takeda S, Takahashi M.
タイトル Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine.
ジャーナル PLoS One
Abstract Regenerative medicine has received a lot of attention as a novel strategy for injuries and diseases that are difficult to cure using current techniques. Cell production, which is vital for regenerative medicine, has undergone remarkable progress via breakthroughs in developmental biology and tissue engineering; currently, cell production requires numerous experimental operators performing manual, small-scale cell cultures. Other major obstacles for cell production and regenerative medicine include the variable quality of products based on the experimental procedure, the skills of operators, the level of labor required for production, and costs. Technological developments are required to overcome this, including automation instead of manual culture. Age-related macular regeneration (AMD) is a refractory ocular disease that causes severe deterioration in central vision due to senescence in the retinal pigment epithelium (RPE). Recently, we performed an autologous transplantation of induced pluripotent stem (iPS) cell-derived RPE cell sheets and started clinical research on allografts from RPE cell suspensions differentiated from iPS cells. The use of regenerative therapies for AMD using iPS cell-derived RPE is expected to become more widespread. In the present study, human iPS cell-derived RPE cells were cultured to form RPE cell sheets using equipment with a closed culture module. The quality of the automated cultured RPE cell sheets was confirmed by comparing their morphological and biological properties with those of manually generated RPE cell sheets. As a result, machine-cultured RPE sheets displayed the same quality as manually cultured RPE sheets, showing that iPS cell-derived RPE cell sheets were successfully cultured by an automated process.
巻・号 14(3)
ページ e0212369
公開日 2019-1-1
DOI 10.1371/journal.pone.0212369
PII PONE-D-18-32290
PMID 30865653
PMC PMC6415881
MeSH Automation, Laboratory Cell Culture Techniques / methods Cells, Cultured Eye Proteins / metabolism Feasibility Studies Humans Immunohistochemistry Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Macular Degeneration / therapy Nerve Growth Factors / metabolism Regenerative Medicine / methods* Retinal Pigment Epithelium / cytology* Retinal Pigment Epithelium / metabolism Serpins / metabolism Tissue Engineering / methods Vascular Endothelial Growth Factor A / metabolism
IF 2.776
引用数 3
リソース情報
ヒト・動物細胞 253G1(HPS0002)