RRC ID |
54938
|
Author |
Horie T, Horie R, Chen K, Cao C, Nakagawa M, Kusakabe TG, Satoh N, Sasakura Y, Levine M.
|
Title |
Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics.
|
Journal |
Genes Dev
|
Abstract |
The CNS of the protovertebrate Ciona intestinalis contains a single cluster of dopaminergic (DA) neurons, the coronet cells, which have been likened to the hypothalamus of vertebrates. Whole-embryo single-cell RNA sequencing (RNA-seq) assays identified Ptf1a as the most strongly expressed cell-specific transcription factor (TF) in DA/coronet cells. Knockdown of Ptf1a activity results in their loss, while misexpression results in the appearance of supernumerary DA/coronet cells. Photoreceptor cells and ependymal cells are the most susceptible to transformation, and both cell types express high levels of Meis Coexpression of both Ptf1a and Meis caused the wholesale transformation of the entire CNS into DA/coronet cells. We therefore suggest that the reiterative use of functional manipulations and single-cell RNA-seq assays is an effective means for the identification of regulatory cocktails underlying the specification of specific cell identities.
|
Volume |
32(19-20)
|
Pages |
1297-1302
|
Published |
2018-10-1
|
DOI |
10.1101/gad.317669.118
|
PII |
gad.317669.118
|
PMID |
30228204
|
PMC |
PMC6169837
|
MeSH |
Animals
Cell Differentiation
Ciona intestinalis / embryology
Ciona intestinalis / genetics*
Ciona intestinalis / growth & development
Ciona intestinalis / metabolism
Dopaminergic Neurons / cytology
Dopaminergic Neurons / metabolism*
Embryo, Nonmammalian / metabolism
Gene Expression Profiling
Gene Regulatory Networks
Single-Cell Analysis
Transcription Factors / metabolism
|
IF |
9.527
|
Times Cited |
7
|
Resource |
C.intestinalis / (O.japonicus) |
Wild C. int
Tg[MiCiTHK]2
pSPeCFP
pSP-Kaede |