Reference - Detail
| RRC ID | 54939 |
|---|---|
| Author | Oonuma K, Kusakabe TG. |
| Title | Spatio-temporal regulation of Rx and mitotic patterns shape the eye-cup of the photoreceptor cells in Ciona. |
| Journal | Dev Biol |
| Abstract |
The ascidian larva has a pigmented ocellus comprised of a cup-shaped array of approximately 30 photoreceptor cells, a pigment cell, and three lens cells. Morphological, physiological and molecular evidence has suggested evolutionary kinship between the ascidian larval photoreceptors and vertebrate retinal and/or pineal photoreceptors. Rx, an essential factor for vertebrate photoreceptor development, has also been suggested to be involved in the development of the ascidian photoreceptor cells, but a recent revision of the photoreceptor cell lineage raised a crucial discrepancy between the reported expression patterns of Rx and the cell lineage. Here, we report spatio-temporal expression patterns of Rx at single-cell resolution along with mitotic patterns up to the final division of the photoreceptor-lineage cells in Ciona. The expression of Rx commences in non-photoreceptor a-lineage cells on the right side of the anterior sensory vesicle at the early tailbud stage. At the mid tailbud stage, Rx begins to be expressed in the A-lineage photoreceptor cell progenitors located on the right side of the posterior sensory vesicle. Thus, Rx is specifically but not exclusively expressed in the photoreceptor-lineage cells in the ascidian embryo. Two cis-regulatory modules are shown to be important for the photoreceptor-lineage expression of Rx. The cell division patterns of the photoreceptor-lineage cells rationally explain the generation of the cup-shaped structure of the pigmented ocellus. The present findings demonstrate the complete cell lineage of the ocellus photoreceptor cells and provide a framework elucidating the molecular and cellular mechanisms of photoreceptor development in Ciona. |
| Volume | 445(2) |
| Pages | 245-255 |
| Published | 2019-1-15 |
| DOI | 10.1016/j.ydbio.2018.11.011 |
| PII | S0012-1606(18)30677-8 |
| PMID | 30502325 |
| MeSH | Animals Animals, Genetically Modified Base Sequence Binding Sites / genetics Cell Lineage / genetics Ciona intestinalis / cytology Ciona intestinalis / genetics* Ciona intestinalis / growth & development* Evolution, Molecular Gene Expression Regulation, Developmental Homeodomain Proteins / genetics* Larva / cytology Larva / genetics Larva / growth & development Mitosis / genetics Photoreceptor Cells, Invertebrate / cytology* Regulatory Sequences, Ribonucleic Acid Spatio-Temporal Analysis |
| IF | 2.936 |
| Times Cited | 1 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.intestinalis / (O.japonicus) | Wild C. int |