RRC ID 54972
Author Shiraishi T, Yokota S, Sato Y, Ito T, Fukiya S, Yamamoto S, Sato T, Yokota A.
Title Lipoteichoic acids are embedded in cell walls during logarithmic phase, but exposed on membrane vesicles in Lactobacillus gasseri JCM 1131T.
Journal Benef Microbes
Abstract Lipoteichoic acid (LTA) is a cell surface molecule specific to Gram-positive bacteria. How LTA localises on the cell surface is a fundamental issue in view of recognition and immunomodulation in hosts. In the present study, we examined LTA localisation using strain JCM 1131T of Lactobacillus gasseri, which is a human intestinal lactic acid bacterium, during various growth phases by immunoelectron microscopy. We first evaluated the specificity of anti-LTA monoclonal antibody clone 55 used as a probe. The glycerophosphate backbone comprising almost intact size (20 to 30 repeating units) of LTA was required for binding. The antibody did not bind to other cellular components, including wall-teichoic acid. Immunoelectron microscopy indicated that LTA was embedded in the cell wall during the logarithmic phase, and was therefore not exposed on the cell surface. Similar results were observed for Lactobacillus fermentum ATCC 9338 and Lactobacillus rhamnosus ATCC 7469T. By contrast, membrane vesicles were observed in the logarithmic phase of L. gasseri with LTA exposed on their surface. In the stationary and death phases, LTA was exposed on cell wall-free cell membrane generated by autolysis. The dramatic alternation of localisation in different growth phases and exposure on the surface of membrane vesicles should relate with complicated interaction between bacteria and host.
Volume 9(4)
Pages 653-662
Published 2018-6-15
DOI 10.3920/BM2017.0124
PMID 29633638
MeSH Antibodies, Monoclonal / metabolism Antigens, Surface / metabolism* Cell Membrane / chemistry Cell Membrane / metabolism* Cell Membrane / ultrastructure Cell Wall / chemistry Cell Wall / metabolism* Lactobacillus fermentum Lactobacillus gasseri / chemistry Lactobacillus gasseri / growth & development Lactobacillus gasseri / physiology* Lactobacillus gasseri / ultrastructure Lactobacillus rhamnosus Lipopolysaccharides / metabolism* Probiotics Protein Binding Teichoic Acids / metabolism*
IF 2.939
Times Cited 0
General Microbes JCM 1131, JCM 1192, JCM 1222