RRC ID 54977
Author Severi KE, Böhm UL, Wyart C.
Title Investigation of hindbrain activity during active locomotion reveals inhibitory neurons involved in sensorimotor processing.
Journal Sci Rep
Abstract Locomotion in vertebrates relies on motor circuits in the spinal cord receiving inputs from the hindbrain to execute motor commands while dynamically integrating proprioceptive sensory feedback. The spatial organization of the neuronal networks driving locomotion in the hindbrain and role of inhibition has not been extensively investigated. Here, we mapped neuronal activity with single-cell resolution in the hindbrain of restrained transgenic Tg(HuC:GCaMP5G) zebrafish larvae swimming in response to whole-field visual motion. We combined large-scale population calcium imaging in the hindbrain with simultaneous high-speed recording of the moving tail in animals where specific markers label glycinergic inhibitory neurons. We identified cells whose activity preferentially correlates with the visual stimulus or motor activity and used brain registration to compare data across individual larvae. We then morphed calcium imaging data onto the zebrafish brain atlas to compare with known transgenic markers. We report cells localized in the cerebellum whose activity is shut off by the onset of the visual stimulus, suggesting these cells may be constitutively active and silenced during sensorimotor processing. Finally, we discover that the activity of a medial stripe of glycinergic neurons in the domain of expression of the transcription factor engrailed1b is highly correlated with the onset of locomotion. Our efforts provide a high-resolution, open-access dataset for the community by comparing our functional map of the hindbrain to existing open-access atlases and enabling further investigation of this population's role in locomotion.
Volume 8(1)
Pages 13615
Published 2018-9-11
DOI 10.1038/s41598-018-31968-4
PII 10.1038/s41598-018-31968-4
PMID 30206288
PMC PMC6134141
MeSH Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / physiology Brain / physiology* Larva / genetics Larva / physiology Locomotion / physiology Motor Activity / genetics Motor Activity / physiology* Neurons / physiology* Psychomotor Performance / physiology Rhombencephalon / physiology Sensorimotor Cortex / physiology* Single-Cell Analysis Spinal Cord / physiology Swimming Zebrafish
IF 4.011
Times Cited 8
Zebrafish Tg(vglut2a:|R|-Gal4) Tg(glyt2:|R|-Gal4) Tg(gad1b:GFP) Tg(vglut2a:loxP-DsRed-loxP-GFP)