論文 - 詳細
| RRC ID | 54979 |
|---|---|
| 著者 | Tabor KM, Marquart GD, Hurt C, Smith TS, Geoca AK, Bhandiwad AA, Subedi A, Sinclair JL, Rose HM, Polys NF, Burgess HA. |
| タイトル | Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping. |
| ジャーナル | Elife |
| Abstract |
Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies. |
| 巻・号 | 8 |
| 公開日 | 2019-2-8 |
| DOI | 10.7554/eLife.42687 |
| PII | 42687 |
| PMID | 30735129 |
| PMC | PMC6392497 |
| MeSH | Animals Animals, Genetically Modified / genetics Brain / physiology Brain / ultrastructure* Brain Mapping / methods* Cell Lineage / genetics DNA-Binding Proteins / genetics Gene Expression Regulation / genetics Integrases / genetics Neuroimaging / methods* Neurons / physiology Neurons / ultrastructure* Transcription Factors / genetics Zebrafish / genetics Zebrafish / physiology |
| IF | 7.551 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(vglut2a:loxP-DsRed-loxP-GFP) |