RRC ID 54979
Author Tabor KM, Marquart GD, Hurt C, Smith TS, Geoca AK, Bhandiwad AA, Subedi A, Sinclair JL, Rose HM, Polys NF, Burgess HA.
Title Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping.
Journal Elife
Abstract Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.
Volume 8
Published 2019-2-8
DOI 10.7554/eLife.42687
PII 42687
PMID 30735129
PMC PMC6392497
MeSH Animals Animals, Genetically Modified / genetics Brain / physiology Brain / ultrastructure* Brain Mapping / methods* Cell Lineage / genetics DNA-Binding Proteins / genetics Gene Expression Regulation / genetics Integrases / genetics Neuroimaging / methods* Neurons / physiology Neurons / ultrastructure* Transcription Factors / genetics Zebrafish / genetics Zebrafish / physiology
IF 7.551
Times Cited 6
Zebrafish Tg(vglut2a:loxP-DsRed-loxP-GFP)