RRC ID 54983
著者 Miyazawa H, Okumura K, Hiyoshi K, Maruyama K, Kakinuma H, Amo R, Okamoto H, Yamasu K, Tsuda S.
タイトル Optical interrogation of neuronal circuitry in zebrafish using genetically encoded voltage indicators.
ジャーナル Sci Rep
Abstract Optical measurement of membrane potentials enables fast, direct and simultaneous detection of membrane potentials from a population of neurons, providing a desirable approach for functional analysis of neuronal circuits. Here, we applied recently developed genetically encoded voltage indicators, ASAP1 (Accelerated Sensor of Action Potentials 1) and QuasAr2 (Quality superior to Arch 2), to zebrafish, an ideal model system for studying neurogenesis. To achieve this, we established transgenic lines which express the voltage sensors, and showed that ASAP1 is expressed in zebrafish neurons. To examine whether neuronal activity could be detected by ASAP1, we performed whole-cerebellum imaging, showing that depolarization was detected widely in the cerebellum and optic tectum upon electrical stimulation. Spontaneous activity in the spinal cord was also detected by ASAP1 imaging at single-cell resolution as well as at the neuronal population level. These responses mostly disappeared following treatment with tetrodotoxin, indicating that ASAP1 enabled optical measurement of neuronal activity in the zebrafish brain. Combining this method with other approaches, such as optogenetics and behavioural analysis may facilitate a deeper understanding of the functional organization of brain circuitry and its development.
巻・号 8(1)
ページ 6048
公開日 2018-4-16
DOI 10.1038/s41598-018-23906-1
PII 10.1038/s41598-018-23906-1
PMID 29662090
PMC PMC5902623
MeSH Action Potentials* Animals Animals, Genetically Modified / genetics Cerebellum / cytology Cerebellum / physiology Gene Expression Membrane Potentials Nerve Net / cytology Nerve Net / physiology* Optogenetics* / methods Spinal Cord / cytology Spinal Cord / physiology Voltage-Sensitive Dye Imaging* / methods Zebrafish / genetics Zebrafish / physiology*
IF 4.011
引用数 8
リソース情報
ゼブラフィッシュ Tg(huC:GVP) Tg(UAS:lyn-RFP) hspGFFDMC28C gSA2AzGFF152B UAS:RFP