RRC ID 54998
著者 Sarangdhar MA, Chaubey D, Srikakulam N, Pillai B.
タイトル Parentally inherited long non-coding RNA Cyrano is involved in zebrafish neurodevelopment.
ジャーナル Nucleic Acids Res
Abstract Transfer of genetic material from parents to progeny via fusion of gametes is a way to ensure flow of information from one generation to the next. Apart from the genetic material, gametes provide a rich source of other factors such as RNA and proteins which can control traits of the embryo. Non-coding RNAs are not only carriers of regulatory information but can also encode memory of events of parental life. Here, we explore the possibility of parental inheritance of non-coding RNAs, especially long non-coding RNAs. Meta-analysis of RNA-seq data revealed several non-coding RNAs present in zebrafish oocyte, sperm and 2cell-stage. The embryo is transcriptionally silent at this stage, we rationalize that all the RNAs detectable at 2cell-stage are deposited either by sperm or oocyte or both and thus inherited. In the inherited pool, we noticed a conserved lncRNA, Cyrano previously known for zebrafish brain development. Knockdown of inherited Cyrano by miR-7 without changing zygotic Cyrano altered brain morphology at 24 hpf and 48 hpf. This defect could be partially rescued by injecting full length Cyrano lncRNA or a mutant resilient to knock-down by miR-7. In future, there is ample scope to check the possibility of inherited lncRNAs as carriers of memory of parental life events and building blocks that set up an initial platform for development.
巻・号 46(18)
ページ 9726-9735
公開日 2018-10-12
DOI 10.1093/nar/gky628
PII 5053168
PMID 30011017
PMC PMC6182166
MeSH Animals Animals, Genetically Modified Brain / embryology* Embryo, Nonmammalian Gene Expression Regulation, Developmental Gene Knockdown Techniques Gene Regulatory Networks High-Throughput Nucleotide Sequencing Inheritance Patterns / genetics MicroRNAs / physiology RNA, Long Noncoding / physiology* RNA, Messenger, Stored / genetics RNA, Messenger, Stored / metabolism Sequence Analysis, RNA Zebrafish / embryology* Zebrafish / genetics*
IF 11.147
引用数 7
リソース情報
ゼブラフィッシュ Tg (Huc:Kaede)