| RRC ID |
54998
|
| Author |
Sarangdhar MA, Chaubey D, Srikakulam N, Pillai B.
|
| Title |
Parentally inherited long non-coding RNA Cyrano is involved in zebrafish neurodevelopment.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
Transfer of genetic material from parents to progeny via fusion of gametes is a way to ensure flow of information from one generation to the next. Apart from the genetic material, gametes provide a rich source of other factors such as RNA and proteins which can control traits of the embryo. Non-coding RNAs are not only carriers of regulatory information but can also encode memory of events of parental life. Here, we explore the possibility of parental inheritance of non-coding RNAs, especially long non-coding RNAs. Meta-analysis of RNA-seq data revealed several non-coding RNAs present in zebrafish oocyte, sperm and 2cell-stage. The embryo is transcriptionally silent at this stage, we rationalize that all the RNAs detectable at 2cell-stage are deposited either by sperm or oocyte or both and thus inherited. In the inherited pool, we noticed a conserved lncRNA, Cyrano previously known for zebrafish brain development. Knockdown of inherited Cyrano by miR-7 without changing zygotic Cyrano altered brain morphology at 24 hpf and 48 hpf. This defect could be partially rescued by injecting full length Cyrano lncRNA or a mutant resilient to knock-down by miR-7. In future, there is ample scope to check the possibility of inherited lncRNAs as carriers of memory of parental life events and building blocks that set up an initial platform for development.
|
| Volume |
46(18)
|
| Pages |
9726-9735
|
| Published |
2018-10-12
|
| DOI |
10.1093/nar/gky628
|
| PII |
5053168
|
| PMID |
30011017
|
| PMC |
PMC6182166
|
| MeSH |
Animals
Animals, Genetically Modified
Brain / embryology*
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Gene Regulatory Networks
High-Throughput Nucleotide Sequencing
Inheritance Patterns / genetics
MicroRNAs / physiology
RNA, Long Noncoding / physiology*
RNA, Messenger, Stored / genetics
RNA, Messenger, Stored / metabolism
Sequence Analysis, RNA
Zebrafish / embryology*
Zebrafish / genetics*
|
| IF |
11.147
|
| Times Cited |
7
|
| Resource |
| Zebrafish |
Tg (Huc:Kaede) |
