論文 - 詳細
| RRC ID | 54998 |
|---|---|
| 著者 | Sarangdhar MA, Chaubey D, Srikakulam N, Pillai B. |
| タイトル | Parentally inherited long non-coding RNA Cyrano is involved in zebrafish neurodevelopment. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Transfer of genetic material from parents to progeny via fusion of gametes is a way to ensure flow of information from one generation to the next. Apart from the genetic material, gametes provide a rich source of other factors such as RNA and proteins which can control traits of the embryo. Non-coding RNAs are not only carriers of regulatory information but can also encode memory of events of parental life. Here, we explore the possibility of parental inheritance of non-coding RNAs, especially long non-coding RNAs. Meta-analysis of RNA-seq data revealed several non-coding RNAs present in zebrafish oocyte, sperm and 2cell-stage. The embryo is transcriptionally silent at this stage, we rationalize that all the RNAs detectable at 2cell-stage are deposited either by sperm or oocyte or both and thus inherited. In the inherited pool, we noticed a conserved lncRNA, Cyrano previously known for zebrafish brain development. Knockdown of inherited Cyrano by miR-7 without changing zygotic Cyrano altered brain morphology at 24 hpf and 48 hpf. This defect could be partially rescued by injecting full length Cyrano lncRNA or a mutant resilient to knock-down by miR-7. In future, there is ample scope to check the possibility of inherited lncRNAs as carriers of memory of parental life events and building blocks that set up an initial platform for development. |
| 巻・号 | 46(18) |
| ページ | 9726-9735 |
| 公開日 | 2018-10-12 |
| DOI | 10.1093/nar/gky628 |
| PII | 5053168 |
| PMID | 30011017 |
| PMC | PMC6182166 |
| MeSH | Animals Animals, Genetically Modified Brain / embryology* Embryo, Nonmammalian Gene Expression Regulation, Developmental Gene Knockdown Techniques Gene Regulatory Networks High-Throughput Nucleotide Sequencing Inheritance Patterns / genetics MicroRNAs / physiology RNA, Long Noncoding / physiology* RNA, Messenger, Stored / genetics RNA, Messenger, Stored / metabolism Sequence Analysis, RNA Zebrafish / embryology* Zebrafish / genetics* |
| IF | 11.147 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg (Huc:Kaede) |