RRC ID 55012
Author Kawahara A, Morita H, Yanagi K, Suzuki H, Mori T, Ohga R, Taimatsu K.
Title Spatiotemporal expression of the cocaine- and amphetamine-regulated transcript-like (cart-like) gene during zebrafish embryogenesis.
Journal Gene Expr Patterns
Abstract The cocaine- and amphetamine-regulated transcript (CART) genes are involved in the neural regulation of energy homeostasis; however, their developmental expressions and functions are not fully understood in vertebrates. We have identified a novel zebrafish cart-like gene that encodes a protein of 105 amino acids possessing sequence similarity to zebrafish and mammalian CART proteins. RT-PCR analysis revealed that the cart-like transcripts were maternally supplied and gradually decreased during the cleavage, blastula and gastrula stages; then, transcripts subsequently reaccumulated at the segmentation, pharyngula and hatching stages. Based on a whole-mount in situ hybridization analysis using an antisense cart-like RNA probe, we found that the cart-like transcript was predominantly expressed in both the Rohon-Beard neurons and trigeminal ganglia, suggesting the involvement of the cart-like gene in zebrafish neural development.
Volume 30
Pages 1-6
Published 2018-12
DOI 10.1016/j.gep.2018.08.002
PII S1567-133X(18)30068-1
PMID 30125742
MeSH Amino Acid Sequence Animals Blastula / cytology Blastula / metabolism Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism* Embryonic Development Gastrula / cytology Gastrula / metabolism Gene Expression Regulation, Developmental* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Neurogenesis Phylogeny Sequence Homology Spatio-Temporal Analysis Zebrafish / embryology* Zebrafish / genetics* Zebrafish / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / metabolism*
IF 1.154
Times Cited 0
Zebrafish Tg (zCREST2-hsp70:GFP)