論文 - 詳細
| RRC ID | 55019 |
|---|---|
| 著者 | Yang YHC, Kawakami K, Stainier DY. |
| タイトル | A new mode of pancreatic islet innervation revealed by live imaging in zebrafish. |
| ジャーナル | Elife |
| Abstract |
Pancreatic islets are innervated by autonomic and sensory nerves that influence their function. Analyzing the innervation process should provide insight into the nerve-endocrine interactions and their roles in development and disease. Here, using in vivo time-lapse imaging and genetic analyses in zebrafish, we determined the events leading to islet innervation. Comparable neural density in the absence of vasculature indicates that it is dispensable for early pancreatic innervation. Neural crest cells are in close contact with endocrine cells early in development. We find these cells give rise to neurons that extend axons toward the islet as they surprisingly migrate away. Specific ablation of these neurons partly prevents other neurons from migrating away from the islet resulting in diminished innervation. Thus, our studies establish the zebrafish as a model to interrogate mechanisms of organ innervation, and reveal a novel mode of innervation whereby neurons establish connections with their targets before migrating away. |
| 巻・号 | 7 |
| 公開日 | 2018-6-19 |
| DOI | 10.7554/eLife.34519 |
| PII | 34519 |
| PMID | 29916364 |
| PMC | PMC6039180 |
| MeSH | Animals Animals, Genetically Modified Biomarkers / metabolism Cell Communication Cell Differentiation Cell Movement Embryo, Nonmammalian Endocrine Cells / cytology Endocrine Cells / physiology* Gene Expression Insulin / genetics Insulin / metabolism Islets of Langerhans / cytology Islets of Langerhans / innervation* Islets of Langerhans / physiology Nerve Net / cytology Nerve Net / physiology* Neural Crest / cytology Neural Crest / physiology* Parasympathetic Nervous System / cytology Parasympathetic Nervous System / physiology* Somatostatin / genetics Somatostatin / metabolism Synaptic Transmission / physiology* Tubulin / genetics Tubulin / metabolism Zebrafish |
| IF | 7.551 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 26 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:GFP |