論文 - 詳細
| RRC ID | 55023 |
|---|---|
| 著者 | Muto A, Kawakami K. |
| タイトル | Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae. |
| ジャーナル | J Vis Exp |
| Abstract |
To identify the role of a subpopulation of neurons in behavior, it is essential to test the consequences of blocking its activity in living animals. Laser ablation of neurons is an effective method for this purpose when neurons are selectively labeled with fluorescent probes. In the present study, protocols for laser ablating a subpopulation of neurons using a two-photon microscope and testing of its functional and behavioral consequences are described. In this study, prey capture behavior in zebrafish larvae is used as a study model. The pretecto-hypothalamic circuit is known to underlie this visually-driven prey catching behavior. Zebrafish pretectum were laser-ablated, and neuronal activity in the inferior lobe of the hypothalamus (ILH; the target of the pretectal projection) was examined. Prey capture behavior after pretectal ablation was also tested. |
| 巻・号 | (136) |
| 公開日 | 2018-6-2 |
| DOI | 10.3791/57485 |
| PMID | 29912192 |
| PMC | PMC6101476 |
| MeSH | Animals Behavior, Animal / physiology* Calcium / chemistry* Larva / physiology* Neurons / physiology* Zebrafish |
| IF | 1.108 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Youtube |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | gSAIzGFFM119B hspGFFDMC76A UAShspzGCaMP6s UAS:EGFP |