RRC ID 55031
Author Makino Y, Jensen NH, Yokota N, Rossner MJ, Akiyama H, Shirahige K, Okada Y.
Title Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression.
Journal Sci Rep
Abstract Gonocyte-to-spermatogonia transition is a critical fate determination process to initiate sperm production throughout the lifecycle. However, the molecular dynamics of this process has not been fully elucidated mainly due to the asynchronized differentiation stages of neonatal germ cells. In this study, we employed single cell RNA sequencing analyses of P1.5-5.5 germ cells to clarify the temporal dynamics of gene expression during gonocyte-to-spermatogonia transition. The analyses identified transcriptional modules, one of which regulates spermatogonial gene network in neonatal germ cells. Among them, we identified Dec2, a bHLH-type transcription factor, as a transcriptional repressor for a spermatogonial differentiation factor Sohlh1. Deficiency of Dec2 in mice induces significant reduction of undifferentiated spermatogonia, and transplantation assay using Dec2-depleted cells also demonstrated the impaired efficiency of engraftment, suggesting its role in maintaining spermatogonial stem cells (SSCs). Collectively, this study revealed the intrinsic role of a new SSC factor Dec2, which protects germ cells from inadequate differentiation during neonatal testis development.
Volume 9(1)
Pages 6063
Published 2019-4-15
DOI 10.1038/s41598-019-42578-z
PII 10.1038/s41598-019-42578-z
PMID 30988352
PMC PMC6465314
MeSH Animals Animals, Newborn Basic Helix-Loop-Helix Transcription Factors / genetics* Cell Differentiation / genetics Gene Expression Regulation, Developmental* Male Mice Mice, Transgenic Single-Cell Analysis Spermatogenesis / genetics* Spermatogonia / physiology* Stem Cells / physiology Testis / cytology Testis / growth & development Transcription Factors / genetics Transcription Factors / metabolism*
IF 4.011
Times Cited 3
DNA material pENTR4-H1 (RDB04395) CS-RfA-EVBsd (RDB06090) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)
Human and Animal Cells 293T(RCB2202)